We narrowed to 9,167 results for: Pol

1. pcDNA3.1-CMV-mCherry-hPMCA2w/b

   Plasmid

   #111570

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYES2/103Q

   Plasmid

   #1385

   Depositor

   Michael Sherman

   Article

   Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

   Insert

   huntingtin (103Q) (HTT Human)
   

   Tags

   EGFP and FLAG

   Expression

   Yeast

   Mutation

   Exon1 sequences containing the first 17 amino aci…

   Available Since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CAG-FLInChR-mVenus

   Plasmid

   #119298

   Purpose

   FLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitor

   Depositor

   Alla Karpova

   Article

   Brown et al Cell. 2018 Oct 16. pii: S0092-8674(18)31

   Insert

   Nx1BTM-FCS-ChR2ET/TC-mVenus
   

   Use

   AAV

   Tags

   mVenus

   Promoter

   CAG

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmRFP-N1 human cofilin S3E

   Plasmid

   #50858

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pGGB_YFP-MiniTurboID

   Plasmid

   #222433

   Purpose

   Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.

   Depositor

   Dominique Bergmann

   Article

   Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

   Insert

   MiniTurboID (BirA mutant)
   

   Use

   Golden gate / green gate compatible cloning vector

   Tags

   Linker and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GfaABC1D BARK D110A p2A mCherry

   Plasmid

   #117692

   Purpose

   GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   AAV

   Tags

   HA / FLAG

   Mutation

   D110A

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGM227

   Plasmid

   #220182

   Purpose

   Expresses exogenous wild-type TOMM70A

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TOMM70A (TOMM70 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS2022

   Plasmid

   #49129

   Purpose

   AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-PRS2/3:Con-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   DBH

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCA13-pHR-SFFV-HaloTag-FBL

   Plasmid

   #199444

   Purpose

   Expression of HaloTag-FBL in mammalian cells

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   FBL (FBL Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

    Plasmid

    #89888

    Purpose

    Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-KRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-C-p50

    Plasmid

    #180238

    Purpose

    Expresses p50 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guo et al Proc Natl Acad Sci U S A. 2016 Sep 6;113

    Insert

    p50 (DCTN2 Human)
    

    Tags

    pmCherry-C1

    Expression

    Mammalian

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAP05

    Plasmid

    #186261

    Purpose

    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Inserts

    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    

    Expression

    Bacterial

    Promoter

    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Tags

    Cerulean

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CaV1.3e[8a,11,31b,Δ32,42a]

    Plasmid

    #49333

    Purpose

    Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    CMV

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV N-term HA-FLAG puro BRD7

    Plasmid

    #41934

    Depositor

    Stephen Elledge

    Article

    Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Retroviral

    Tags

    HA-Flag

    Expression

    Mammalian

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pYES2/25Q

    Plasmid

    #1384

    Depositor

    Michael Sherman

    Article

    Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

    Insert

    huntingtin (25Q) (HTT Human)
    

    Tags

    EGFP and FLAG

    Expression

    Yeast

    Mutation

    Exon1 sequences containing the first 17 amino aci…

    Available Since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMT-Mef2c-tPT2A-GFP

    Plasmid

    #111771

    Purpose

    Bicistronic construct: Co-express two genes by 2A peptides

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Insert

    Mef2c-tPT2A-GFP (Mef2c Mouse)
    

    Use

    Cloning intermediate

    Tags

    GFP

    Promoter

    T7

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG-Crx-IRES-GFPd2

    Plasmid

    #73997

    Purpose

    Plasmid expressing Crx-IRES- GFPd2

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Crx-IRES-GFPd2 (Crx Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWZL Blast Snail ER

    Plasmid

    #18798

    Depositor

    Bob Weinberg

    Article

    Mani et al Cell. 2008 May 16. 133(4):704-15.

    Insert

    snail (SNAI1 Human)
    

    Use

    Retroviral

    Tags

    ER hormone binding domain

    Expression

    Mammalian

    Mutation

    G525R mutation in the ER. The presence of the G52…

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRMC2-sec:msfGFP

    Plasmid

    #194914

    Purpose

    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Expression

    Bacterial

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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