We narrowed to 32 results for: Pol

In this blog post, we explain gamma-retrovirus packaging systems for those interested in using gamma-retroviral systems in the lab.

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

Addgene lentiviral packaging, envelope, and transfer plasmids

Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids.

Protocol for Ligation Independent Cloning

Addgene answers questions about lentiviral plasmids, including 2nd vs 3rd generation transfer vectors, packaging plasmids, biosafety concerns, and more.

Discover the tools you need to perform plant genome engineering with CRISPR.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

This post describes the development of sentinel plants, which change color to alert humans when they detect certain molecules in their environment.

Polyclonal antibodies are a common research reagent - learn how they’re produced and the advantages and disadvantages of using them in your next experiment.

Tips to consider when publishing a scientific manuscript, including what type of journal to publish in, peer review, and helpful dos and don'ts.

The basics of PCR covering end point PCR, qPCR, ddPCR, and multiplex PCR and how each method is used.

Thinking about a career in science policy? In this article, we cover the who, what, when, where, why, and how of science policy careers.

We're updating our Hold for Publication policy. Read about how these changes will help streamline our workflows and get your plasmids online faster!

Description of the components of the 4 most common viruses - AAV, adenovirus, lentivirus, and gamma-retrovirus.

Learn more about non-homologous end joining (NHEJ) and how it relates to genome engineering technologies such as CRISPR/Cas9.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Overview of virus-specific safety controls as well as general safety procedures for viral handling.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Fragmid is a quick, easy way to design and create CRISPR plasmids. Read about the Doench lab's new modular cloning-based approach in this blog post!