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Showing: 1 - 20 of 43 results
  1. Lentivirus Plasmids

    Type
    Collection
    ... 12251 pMDLg/pRRE 3rd Packaging Contains Gag and Pol; can be used with 3rd generation transfer plasmids...Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation...12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation...8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag, Pol, Rev, Tat, Vif, and Nef; can be used with 2nd and... Kasparov 22500 pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd Packaging part...part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco N/A Envelope Ecotropic...Reiser 17531 pCD/NL-BH*DDD 2nd Packaging Contains Gag/Pol, Tat, Rev. Use with envelope plasmids from Jakob ...
  2. Retrovirus Plasmids

    Type
    Collection
    ...Contains gag and pol; Use with envelope plasmid Weinberg 12371 pCL-Eco Packaging Contains gag, pol, & env Verma...
  3. Open Enzyme Collection

    Type
    Collection
    ...pOpen-T4gene43 T4 DNA Polymerase 165532 pOpen-ECOpolA E. coli DNA Polymerase I 165533 pOpen-TthpolA Tth DNA polymerase... T7 DNA Polymerase (unmodified) 165542 pOpen-KTHEAQpol KlenTaq1 polymerase 165543 pOpen-Bstpol Bst DNA...Fragment 165562 pOpen-Pwopol Pwo DNA Polymerase 165563 pOpen-BsupolLF Bsu DNA Polymerase I, Large Fragment... DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase 165501...165501 pOpen-GBDpol Pyrococcus sp. Heat-Stable DNA Polymerase 165502 pOpen-9N7polA (CT) 9 degrees N-7 DNA...DNA Polymerase chain terminating 165511 pOpen-K12polLF DNA Polymerase I, Large (Klenow) Fragment 165522...pOpen-K12polLF (exo-) DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-) 165512 pOpen-THEAQpolA Taq ...
  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...His, TEV polH Parkinson's Michael J Fox Foundation MJFF 40375 pBACgus/LRRK2 C-His LRRK2 His polH Parkinson's...His polH Parkinson's Michael J Fox Foundation MJFF 40379 pBACgus/LRRK2 C-His_T2031E LRRK2 His polH Parkinson's...His polH Parkinson's Michael J Fox Foundation MJFF 40381 pBACgus/LRRK2 C-His_G2019S LRRK2 His polH Parkinson's... LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40385 pBACgus4x/Parkin PRKN polH Parkinson's...GST polH Parkinson's Michael J Fox Foundation MJFF 40407 LRRK2_1867_2143 LRRK2 His, TEV, GST polH Parkinson's...GST polH Parkinson's Michael J Fox Foundation MJFF 40409 LRRK2_1867_2134 LRRK2 His, TEV, GST polH Parkinson's... polH Parkinson's Michael J Fox Foundation MJFF 40505 pEMB018 / LRRK2_BC_1309_2187 LRRK2 His polH Parkinson's...
  5. Plasmids for Stem Cell Research

    Type
    Collection
    ...Single polycistronic lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral...27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression of human...(8):1221-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for...Non-integrating mini-intronic plasmid (MIP) polycistronic expression of human Oct4, Klf4, Sox2, c-Myc ...episome Human Non-integrating EBNA1-mediated polycistronic expression of human Sox2, KLF4, L-Myc, Lin28...Replicating EBNA1 episome Human Non-integrating polycistronic expression of human Oct4, Klf4, Sox2, c-Myc,...episome Human Non-integrating EBNA1-mediated polycistronic expression of human Oct4, Sox2, Myc, Klf4, BCL2L1...
  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma polypeptide PI3CG, PI3K...gonadotropin, beta polypeptide CGB3, hCGB CGB1 chorionic gonadotropin, beta polypeptide 1 - CGB2 chorionic... gonadotropin, beta polypeptide 2 - CGB5 chorionic gonadotropin, beta polypeptide 5 HCG, MGC119822 CGB7... beta polypeptide 7 CG-beta-a, FLJ35403, FLJ43118 CGB8 chorionic gonadotropin, beta polypeptide 8 - CHGA... - GIP gastric inhibitory polypeptide - GIPR gastric inhibitory polypeptide receptor MGC126722 GKN1 gastrokine...factor alpha polypeptide PDGF-A, PDGF1 PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma...
  7. Antibody Guide

    Type
    Collection
    ...lower background signals than polyclonal antibodies. However, polyclonal antibodies are more sensitive...clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that are specific... antibodies are typically more expensive than polyclonal antibodies. Environmental factors, binding partners...available epitope on your protein of interest. Polyclonal antibodies are typically collected directly from...targeting various epitopes on the same antigen. Polyclonal antibodies are often more environmentally stable...protein in any form it may be present in. However, polyclonals can vary significantly from lot to lot, as immune...consider the following questions: Do you need polyclonal or monoclonal antibodies? Will you be using a...
  8. Optogenetics AAV Preps

    Type
    Collection
    ...Bruchas Bidirectional: BiPOLES 154948 CaMKII-somBiPOLES-mCerulean CamKII BiPOLES (soma-targeted) mCerulean... 5, 9, rg* Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn BiPOLES (soma-targeted) mCerulean Cre-dependent...variants Jaws stGtACR iC++ OPN3 PPO Bidirectional BiPOLES Promoter CAG CaMKII CBA Dlx EF1a/nEF Synapsin E2...
  9. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... PCR and surveyor. I used DNA polymerase Takara Ex Taq ™ DNA Polymerase for my genomic PCR, but couldn't...In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well. Some groups have ...your HR by doing Restriction Fragment Length Polymorphism (RFLP) ( see Figure 4 of the Cong, et. al, 2013...
  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Marchantia polymorpha MpU6 none S. pyogenes Hara-Nishimura pMpGE_En03 71535 Other/Marchantia polymorpha MpU6...pyogenes Ventura pCRISPRyl 70007 Other/Yarrowia lipolytica SCR1'-tRNA yes, cut S. pyogenes LEU2 Wheeldon...pyogenes Draetta pCAS1yl 73226 Other/Yarrowia lipolytica yes, cut S. pyogenes LEU2 Yang pLCKO 73311 Mammalian...Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 ...in a single vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG ...
  11. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Ligation Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated...acceptable. The bulk sorted cells are composed of a polyclonal population exposed to sgRNA-A and sgRNA-B (see... band” in separate reactions. NOTE: Numerous polymerases may be used for step 6.3 . Run samples in a thermocycler...a single reaction. Optimize multiplexing in a polyclonal population ( i.e., bulk sorted cells) before ...biallelic deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning...
  12. Genetic Code Expansion

    Type
    Collection
    ...aminotyrosine Mammalian Huiwang Ai 157925 pMAH-POLY-eRF1(E55D) POLY-eRF1(E55D) E. coli para-substituted phenylalanine...proteins for various purposes from therapeutics to biopolymers. Tips for Success Before beginning to reprogram...Synthetase Origin ncAA Expression Codon PI 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG...
  13. Viral Production

    Type
    Collection
    ...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...centrifugation and then by filtration through a 0.45 µm polyethersulfone membrane. The preparations are then aliquoted... 293T cell line in the presence of 10.0 μg/mL polybrene. This ensures that any loss of titer associated...
  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Plasma membrane targeted GCaMP6s calcium sensor A polarized Ca(2+), diacylglycerol and STIM1 signalling system... Science. 2017 Nov 3;358(6363):623-630. Franck Polleux Calcium Astrocyte AAV-mediated expression of jRCaMP1a...Encoded Glutamate Indicators with Altered Color and Topology. ACS Chem Biol. 2018 Jan 22. Robert Campbell Glutamate...exploiting a temperature-responsive elastin-like polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai...
  15. Microbiology Resources

    Type
    Collection
    ...vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors...
  16. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Adeno X shuttle vector with EF1 promoter and SV40 polyA pICPIS-CB 73356 AdenoX shuttle vector with chicken-beta...chicken-beta actin CMV enhancer promoter SV40 polyA Lentiviral Transfer Vectors Adenoviral Vectors Return...
  17. Ras Pathway

    Type
    Collection
    ...2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3...containing protein 1 SCRIB Scribbled planar cell polarity protein SHC SHC1 SHC2 SHC3 SHC4 SHC (Src homology...
Showing: 1 - 20 of 43 results