We narrowed to 18,759 results for: nls
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Plasmid#234523PurposeTranscriptional reporter for detecting calcium increase in HEK293T (transcription factor)DepositorInsertCD4-HA-CIBN-MK2-hLOV-TEVcs-GAL4
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceApril 3, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR_CMV_Melt-32-mCherry
Plasmid#234062PurposeEncodes a Melt variant with a switching temperature of 32°CDepositorInsertMelt-32-mCherry
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR_CMV_MeltCasp1-37-GFP
Plasmid#234069PurposeEncodes the caspase1 without CARD domain (with R391K mutation) controlled by a Melt variant with a switching temperature of 37°CDepositorInsertMeltCasp1-37-GFP
UseLentiviralExpressionMammalianMutationMelt(C292A,Q355N); hcaspase1(R391K)PromoterCMVAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459_L31_CRISPR_downstream_sgRNA
Plasmid#235610PurposeEncodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31DepositorInsertRpl31 C-terminus downstream sgRNA
UseCRISPRAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459_L31_CRISPR_upstream_sgRNA
Plasmid#235609PurposeEncodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31DepositorInsertRpl31 C-terminus upstream sgRNA
UseCRISPRAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA
Plasmid#233045PurposeTo express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.DepositorInsertRfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
TagsmCherryExpressionMammalianAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP
Plasmid#196988PurposeDerived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vectorDepositorInsertNestin-dCas9-KRAB-T2a-GFP
UseCRISPR and LentiviralAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2 - sgAP2S1 #2
Plasmid#202757PurposeEncodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1DepositorInsertgRNA targeting AP2S1
UseCRISPR and LentiviralExpressionMammalianAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2 - sgAP2M1
Plasmid#202758PurposeEncodes Doxycycline inducible Cas9 and sgRNA targeting AP2M1DepositorInsertgRNA targeting AP2M1
UseCRISPR and LentiviralExpressionMammalianAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2 - sgAP2S1 #1
Plasmid#202756PurposeEncodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1DepositorInsertgRNA targeting AP2S1
UseCRISPR and LentiviralExpressionMammalianAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgPnpt1-1#
Plasmid#234769Purposeknockout Pnpt1DepositorInsertPnpt1 (Pnpt1 )
UseLentiviralAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYI2206
Plasmid#228348PurposeConstitutive rPhlTA mutant expressionDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT957
Plasmid#228293PurposeConstitutive mutant PhlTA expression with strong promoterDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationE41GPromoterScPGK1 promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1035
Plasmid#228300PurposeConstitutive expression of LuxTA mutant with strong promoterDepositorInsertluxTA mutant
UseSynthetic BiologyTagsNuclear localization signal-Three copies of VP16 …ExpressionYeastMutationS116Y, W201RPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX330-sgRNA006_CTCF
Plasmid#232928PurposeExpression vector for a sgRNA against the mouse CTCF locus and SpCas9.DepositorPublicationAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459-sgRNA048_Grb10
Plasmid#232934PurposeExpression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9-T2A-PuroR (Grb10 S. pyogenes)
ExpressionMammalianAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOG-Cre
Plasmid#233265PurposePlasmid expressing Cre recombinase that is used to insert DNA CARGO into RMCE homing locus in Rosa26DepositorInsertCre recombinase
UseCre/LoxExpressionMammalianPromoterCMVAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051d_sgCiPELO #1
Plasmid#229017PurposeExpression of CRISPRi PELO sgRNA #1DepositorAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7_Sr62NLRa
Plasmid#233528PurposeExpresses Sr62NLRa in yeastDepositorInsertSr62NLRa
TagsGAL4 AD, HA tagExpressionYeastPromoterADH1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.minBG-CI-SaCas9-W3SynpA
Plasmid#231369PurposeminBG-driven SaCas9, with short synthetic terminator sequence.DepositorInsertSaCas9
UseAAVAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.minCMVe-SCP1-SaCas9-W3SynpA
Plasmid#231370PurposeSCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.DepositorInsertSaCas9
UseAAVAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR
Plasmid#216664PurposeExpressed Gal4-inducible ETV2 and BFP, with hygromycin resistanceDepositorInsertsUseLentiviralAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RTN3-WPRE-UbC-Emerald
Plasmid#233467PurposeLentiviral vector plasmid expressing human reticulon 3 (RTN3) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVT-Bxb1
Plasmid#232746PurposeIVT template for making modRNA encoding Bxb1DepositorInsertBxb1
UseIn vitro transcriptionTagsHA and SV40 NLSAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX459_MCM2_sgRNA
Plasmid#232730PurposeGenerates MCM2 dTAG C ternimal knock-in cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMCM2 (MCM2 Human)
ExpressionBacterial and MammalianMutationNOAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX459_GINS4_gRNA
Plasmid#232734PurposeGenerates GINS4 dTAG C ternimal knock-in cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGINS4 (GINS4 Human)
ExpressionBacterial and MammalianMutationNOAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX459_CDC45_sgRNA
Plasmid#232732PurposeGenerates CDC45 dTAG C ternimal knock-in cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCDC45 (CDC45 Human)
ExpressionBacterial and MammalianMutationNOAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP2047 - pAAV-AiE2538m-minBG-iCre(R297T)-BGHpA
Plasmid#220726PurposeAiE2538m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cellsDepositorInsertiCre(R297T)
UseAAVPromoterminBGAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP1788 - pAAV-AiE2116m-minBG-iCre(R297T)-BGHpA
Plasmid#220713PurposeAiE2116m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cellsDepositorInsertiCre(R297T)
UseAAVPromoterminBGAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-hfCas13d-HA-mCherry-pA
Plasmid#233029PurposeTo Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoterDepositorInserthfCas13d-mCherry
UseAAVTagsmCherryAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa
Plasmid#233033PurposeTo Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genomeDepositorInsertDjCas13d and mCherry
UseAAVTagsmCherryAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DjCas13d-HA-mCherry-pA
Plasmid#233030PurposeTo Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoterDepositorInsertDjCas13d-mCherry
UseAAVTagsmCherryAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-CasRx-HA-mCherry-pA
Plasmid#233028PurposeTo Express HA tagged CasRX-mCherry fusion protein from the mammalian EFS promoterDepositorInsertCasRx-mCherry
UseAAVTagsmCherryAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCY5k
Plasmid#160294PurposeYeast CRISPR plasmid targeting the kanMX cassetteDepositorInsertssgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9
UseCRISPRTags8xHis-tag and SV40 NLSAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
EHMT1_exon 3_gRNA
Plasmid#228809PurposeCRISPR targeting of human EHMT1DepositorAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_CAG-TurboGFP
Plasmid#229781PurposeBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFPDepositorInsertTurboGFP
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterCAGAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-CasRx-P2A-mCherry-pA
Plasmid#233025PurposeTo Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A siteDepositorInsertCasRx/mCherry
UseAAVTagsmCherryAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa
Plasmid#233034PurposeTo Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genomeDepositorInserthfCas13d and mCherry
UseAAVTagsmCherryAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGAPDH-Cre-HYG
Plasmid#231510PurposeExpresses Cre recombinase in AcanthamoebaDepositorInsertCre recombinase
UseUnspecified; Acanthamoeba castellanii neff strainTagsEGFP and SV40 NLSPromoterAcanthamoeba GAPDHAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HaloTag-LacI
Plasmid#232636Purposelac repressor (LacI) which binds lacO sites; labeled with HaloTagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHaloTag-LacI
TagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only