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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,448 results for: nls

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Showing: 5901 - 5920 of 6448 results

  1. L4663

    Plasmid
    #1665
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, SV40NLS, gfpN, IVSR, gfpY66W,…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1665-plasmid-map-sequence-id-275

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  2. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid
    #242660
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242660-plasmid-map-sequence-id-489092

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  3. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  8. rnLbCas12a-PE2

    Plasmid
    #213746
    Purpose
    For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Insert
    rnLbCas12a-M-MLV RT∆RNase H
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R, H759A, R1138A in LbCas12a
    Promoter
    CMV
    Available Since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213746-plasmid-map-sequence-id-422194

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  13. pPD16_43

    Plasmid
    #1433
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1433-plasmid-map-sequence-id-44

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  14. pBK125

    Plasmid
    #1688
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    hsp16-25', hsp16-415', SV40NLS, gfpN, g…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1688-plasmid-map-sequence-id-298

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  16. pH-ABE8e-spG

    Plasmid
    #200775
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9-spG(D10A)-TadA8e
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200775-plasmid-map-sequence-id-399599

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  20. pPD93_97

    Plasmid
    #1476
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, IVSA, SV40NLS, gfpN, IVSR, gf…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1476-plasmid-map-sequence-id-87

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Showing: 5901 - 5920 of 6448 results