We narrowed to 13,163 results for: CAR

1. pCDH-EF1a-tetR-T2A-iRFP

   Plasmid

   #176028

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   iRFP
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV123

   Plasmid

   #63189

   Purpose

   pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. -962 human cyclin D1 promoter AP-1 site mutant pGL3Basic

   Plasmid

   #32728

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 promoter (CCND1 Human)
   

   Use

   Luciferase; Luciferase reporter gene

   Mutation

   -947 AP-1 binding site TGAGTCA deletion

   Promoter

   -962 CCND1 promoter

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pAV106

   Plasmid

   #63196

   Purpose

   pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSyn-GW-dCas9-SET2-IRES2-mCherry

   Plasmid

   #202089

   Purpose

   Neuron-specific expression vector of dCas9-Set2

   Depositor

   Elizabeth Heller

   Article

   Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

   Insert

   dCas9-Set2
   

   Tags

   3x FLAG and mCherry

   Expression

   Mammalian

   Promoter

   human Synapsin

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSMP-MBD3_1

   Plasmid

   #36371

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   MBD3 (MBD3 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pVPS13D-I-EGFP

   Plasmid

   #176464

   Purpose

   express internally GFP-tagged VPS13D in mammalian cells

   Depositor

   Richard Youle

   Article

   Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   VPS13D (VPS13D Human)
   

   Tags

   GFP-Ub

   Expression

   Mammalian

   Mutation

   EYFP is internally inserted

   Promoter

   CMV

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-ATG13

   Plasmid

   #171413

   Purpose

   For the purifcation of human ULK1 complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ATG13 (ATG13 Synthetic)
   

   Expression

   Mammalian

   Available Since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV124

   Plasmid

   #63190

   Purpose

   pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUASt-GBP-Trbo

    Plasmid

    #179996

    Purpose

    Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.

    Depositor

    Graydon Gonsalvez

    Article

    Baker et al Development. 2021 Nov 15;148(22). pii: 2

    Insert

    GBP nanobody
    

    Tags

    Trbo

    Expression

    Insect

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcGP67A-rCL1-HormR-GAIN

    Plasmid

    #37847

    Depositor

    Axel Brunger

    Article

    Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

    Insert

    CL1 (Adgrl1 Rat)
    

    Use

    Baculovirus transfer vectors

    Tags

    8xHIS

    Mutation

    HormR and GAIN domains only (aa460–849

    Promoter

    Polyhedrin

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pACYC-Cas12g1-noncoding

    Plasmid

    #120880

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-Cas12g1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

    Plasmid

    #119074

    Purpose

    Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:MCP-p65-HSF1-2a-Cerulean
    

    Expression

    Bacterial

    Promoter

    Zebrafish ubiquitin promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

    Plasmid

    #119075

    Purpose

    Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:Com-KRAB-2a-Cerulean
    

    Expression

    Bacterial

    Promoter

    Zebrafish ubiquitin promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVC-Ds-DrU6a:sgRNA-com-Ds

    Plasmid

    #119077

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xCom-sgRNA
    

    Expression

    Bacterial

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVC-Ds-DrU6a:sgRNA-MS2-Ds

    Plasmid

    #119076

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xMS2-sgRNA
    

    Expression

    Bacterial

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C-SLC35B2

    Plasmid

    #155037

    Purpose

    Transient expression of EGFP-SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-6p-hDcp2

    Plasmid

    #72216

    Purpose

    bacterial expression of GST-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F146Y and E198K (please see depositor's comm…

    Promoter

    tac

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-syn-IRES-hrGFP

    Plasmid

    #107549

    Purpose

    high transduction efficiency AAV-mediated synapsin promoter-dependent expression IRES-hrGFP

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Type

    Empty backbone

    Use

    AAV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Plasmid

    #163074

    Purpose

    Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP1-iEER
    

    Use

    Gateway entry vector

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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