We narrowed to 13,324 results for: GAN

1. JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry)

   Plasmid

   #233545

   Purpose

   A CMV driven human MAFB followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   MAFB (MAFB Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. JDW 1473 (pLenti-BLAST-ZIC3-P2A-H2A-mCherry)

   Plasmid

   #233547

   Purpose

   A CMV driven human ZIC3 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   ZIC3 (ZIC3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. empty_pK7fWG2_IVD_FP611

   Plasmid

   #228371

   Purpose

   Empty dual expression vector for determining subcellular localization for protein of interest

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Type

   Empty backbone

   Use

   Tags

   Green Florescent Protien

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pACEBac1_Strep-Psc-BICD2(1-400)

   Plasmid

   #228830

   Purpose

   Insect cell expression of N-terminal portion of BICD2 (aa 1-400)

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   aa 1-400

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACEBac1_Strep-Psc-BICD2(1-710)

   Plasmid

   #228831

   Purpose

   Insect cell expression of truncated BICD2 (aa 1-710)

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   aa 1-710

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. T2A GFP H2B

   Plasmid

   #227722

   Purpose

   Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Haosheng Sun, Oliver Hobert

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   (nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   T2A 3xNLS GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLigify_dRibose

   Plasmid

   #215561

   Purpose

   Expresses GFP in response to D-Ribose

   Depositor

   David Ross, Simon d'Oelsnitz

   Article

   d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

   Inserts

   GFP mut2
   LcLacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P250 and Plclaci

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKanB

   Plasmid

   #223523

   Purpose

   Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

   Depositor

   Geoff Lin-Cereghino

   Article

   Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Sept. 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. RcSxph

   Plasmid

   #194018

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTarget-Dual

    Plasmid

    #207884

    Purpose

    Carries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.

    Depositor

    David Edgell

    Article

    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

    Inserts

    I-OnuI target site
    I-GpeMI target site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSET-Cm-CspB

    Plasmid

    #215400

    Purpose

    Cm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)

    Depositor

    Gitta Coaker

    Article

    Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

    Insert

    cspB
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSET-Cm-CspA2

    Plasmid

    #215399

    Purpose

    Cm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)

    Depositor

    Gitta Coaker

    Article

    Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

    Insert

    cspA2
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSN667

    Plasmid

    #219963

    Purpose

    Expressed sfGFP fused human KIF1Bß(1-721) in insect cells

    Depositor

    Shinsuke Niwa

    Insert

    codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
    

    Use

    Tags

    sfGFP::2xStrepII tag

    Expression

    Insect

    Mutation

    encodes 1-721

    Promoter

    polyhedrin

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSN668

    Plasmid

    #219962

    Purpose

    Expressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cells

    Depositor

    Shinsuke Niwa

    Insert

    codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
    

    Use

    Tags

    sfGFP::2xStrepII tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RT-5

    Plasmid

    #220442

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (puf-3 Nematode)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ClhN-pADH3-GFP-Pex3-4V5-APEX2-URA

    Plasmid

    #207064

    Purpose

    Expressing GFP-Pex3-4V5-APEX2 under ADH3 promoter. Insufficient signal for EM.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Pex3
    

    Use

    Tags

    4xV5-APEX2 and GFP

    Expression

    Yeast

    Mutation

    Promoter

    pADH3

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS77

    Plasmid

    #215681

    Purpose

    Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ClhN-pADH3-Snf7-4V5-APEX2-URA

    Plasmid

    #207065

    Purpose

    Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Snf7
    

    Use

    Tags

    4xV5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pADH3

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid

    #202586

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Arginine 261 to Alanine

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid

    #202588

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, changed O…

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge