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ApoE4_47-300_mCh-SspB (pBS1069)

Plasmid

#185292

Purpose

For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4_47-300

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE2_47-300_mCh-SspB (pBS1068)

Plasmid

#185291

Purpose

For the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2_47-300

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-bleo-dEgf

Plasmid

#173840

Purpose

A knock-out vector for the dog Egf

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-puro-dHbegf

Plasmid

#173841

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-dEreg

Plasmid

#173843

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-aACry::EGFP-zebrafish Plaat1 (WT)

Plasmid

#168772

Purpose

Express EGFP-zebrafish Plaat1 (WT) in zebrafish lens

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls (plaat1 Zebrafish)

Tags

EGFP

Promoter

alphaA-crystallin promoter

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_A893E-talin1 (1-2541)

Plasmid

#166116

Purpose

For expression of full-length mouse talin-1 carrying mutation A893E in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

A893E-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

A893E

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_L1539P-talin1 (1-2541)

Plasmid

#166119

Purpose

For expression of full-length mouse talin-1 carrying mutation L1539P in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

L1539P-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

L1539P

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_L2509P-talin1 (1-2541)

Plasmid

#166123

Purpose

For expression of full-length mouse talin-1 carrying mutation L2509P in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

L2509P-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

L2509P

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_S1750F-talin1 (1-2541)

Plasmid

#166120

Purpose

For expression of full-length mouse talin-1 carrying mutation S1750F in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

S1750F-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

S1750F

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_D2086V-talin1 (1-2541)

Plasmid

#166122

Purpose

For expression of full-length mouse talin-1 carrying mutation D2086V in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

D2086V-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

D2086V

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_Y1389C-talin1 (1-2541)

Plasmid

#166118

Purpose

For expression of full-length mouse talin-1 carrying mutation Y1389C in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

Y1389C-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

Y1389C

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRAP2_SH3

Plasmid

#110016

Purpose

Protein expression and purification of GRAP2_SH3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GRAP2_SH3 (GRAP2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ABCE1_ABC-tran

Plasmid

#109853

Purpose

Protein expression and purification of ABCE1_ABC-tran

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ABCE1_ABC-tran (ABCE1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr1508

Plasmid

#136746

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr1508

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr1508 (Or4e1 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr288

Plasmid

#136749

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr288

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr288 (Olfr288 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr1395

Plasmid

#136731

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr1395

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr1395

Use

In situ probe

Mutation

ISH Probe insert, D175G and L220F- please dep. co…

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI177

Plasmid

#126077

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP1-10

Use

Binary, module 1

Tags

linker (GTGGGGS)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pVR

Plasmid

#84718

Purpose

E. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilis

Depositor

Nils Walter

Article

Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

Inserts

E. coli RecA promoter
5' UTR of queC gene

Expression

Bacterial

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2235

Plasmid

#111888

Purpose

High copy number plasmid containing Ple320 (POU4F1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

Unspecified

Promoter

Ple320 (POU4F1 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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