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p{3P3-dsRed::FRT,attB}

Plasmid

#182176

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-GFP::KD-RC,attB}

Plasmid

#182184

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-eGFP::FRT,attB}

Plasmid

#182174

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::FRT,attB}

Plasmid

#182186

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::KD-RC,attB}

Plasmid

#182189

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-dsRed::KD-RC,attB}

Plasmid

#182179

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-eGFP::KD,attB}

Plasmid

#182183

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::KD,attB}

Plasmid

#182188

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-mTurquoise::KD,attB}

Plasmid

#182182

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-C4A,C5A,C7A-GFP

Plasmid

#166838

Purpose

Expresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7Ala

Depositor

Daniel Klionsky

Publication

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

C4A, C5A, C7A

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-G2A-GFP

Plasmid

#166837

Purpose

Expresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2Ala

Depositor

Daniel Klionsky

Publication

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

G2A

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI733 pRPR1(TetO)-RPC31-sgRNA

Plasmid

#164913

Purpose

For yeast genomic integration of sgRNA against RPC31

Depositor

Nicholas Ingolia

Publication

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-RPC31-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI732 pRPR1(TetO)-HTS1-sgRNA

Plasmid

#164912

Purpose

For yeast genomic integration of sgRNA against HTS1

Depositor

Nicholas Ingolia

Publication

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-HTS1-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCSJ182

Plasmid

#159723

Purpose

Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Publication

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

GID4 (VID24 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBTMcC24-DM

Plasmid

#111236

Purpose

yeast expression vector

Depositor

Ulrich Stelzl

Publication

Woodsmith et al Nat Methods. 2017 Dec;14(12):1213-1221.

Insert

ccdB-cassette

Expression

Yeast

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRPL28 SpHIS5 (pNTI663)

Plasmid

#115433

Purpose

Expresses wild-type RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Publication

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRPL28(Q38P) SpHIS5 (pNTI664)

Plasmid

#115434

Purpose

Expresses Q38P RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Publication

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

Q38P; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRPL28(H39L) SpHIS5 (pNTI665)

Plasmid

#115435

Purpose

Expresses H39L RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Publication

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

H39L; MfeI site in intron

Promoter

ashbya gospii TEF1 and endogenous

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCBDU-JW

Plasmid

#111237

Purpose

yeast expression vector

Depositor

Ulrich Stelzl

Publication

Woodsmith et al Nat Methods. 2017 Dec;14(12):1213-1221.

Insert

ccdB-cassette

Expression

Yeast

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac-GS

Plasmid

#47769

Purpose

expresses AmTrac-GS in yeast

Depositor

Wolf Frommer

Publication

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-GS

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only