We narrowed to 13,330 results for: GAN

1. ZJOM68

   Plasmid

   #133657

   Purpose

   Integration of TGL3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-TGL3-mCherry (TGL3 Budding Yeast)
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ExRai-CKAR

   Plasmid

   #118409

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-CKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZJOM119

   Plasmid

   #133661

   Purpose

   Integration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-NAB2-mTagBFP2 (NAB2 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. linker-mCitrine-PIF6-NAT

   Plasmid

   #51576

   Purpose

   Tag protein of interest with PIF6 in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PIF6 (PIL2 Mustard Weed)
   

   Use

   Tags

   linker and mCitrine

   Expression

   Yeast

   Mutation

   1-100aa of PIF6

   Promoter

   none

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV BARK p2A mCherry

   Plasmid

   #117689

   Purpose

   CMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs peptide
   

   Use

   Tags

   HA / FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-cytoEKAR4

   Plasmid

   #174438

   Purpose

   Cytosol-targeted EKAR4.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   cytoEKAR4
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PhyB-mCherry-PEX3

   Plasmid

   #51572

   Purpose

   Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   PEX3 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-LiMETER-v2

    Plasmid

    #113934

    Purpose

    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    LOV2 and Stim1 polybasic domain (PB)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-pmEKAR4

    Plasmid

    #174440

    Purpose

    Plasma membrane-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

    Plasmid

    #89347

    Purpose

    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    Jurkat TCRalpha/beta alleles and CD3e and CD3z
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. ZJOM149

    Plasmid

    #133646

    Purpose

    Integration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ELO3-mCherry (ELO3 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV6M-Pak1 K299R

    Plasmid

    #12210

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Catalytically inactive Pak1: K299R

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCXMG

    Plasmid

    #204409

    Purpose

    Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

    Depositor

    David Ackerley

    Article

    Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tol2 8xSTAR-mScarletI-NLS. PGK-puro

    Plasmid

    #136263

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-TRE-SHH

    Plasmid

    #134350

    Purpose

    Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

    Depositor

    Lorenz Studer

    Article

    Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

    Insert

    SHH (SHH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry N1 TOMM22

    Plasmid

    #157762

    Purpose

    Expression of TOMM22:mcherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    TOMM22 (TOMM22 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

    Plasmid

    #87694

    Purpose

    Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Cre-PhoCl-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUASTattB-dALFANb(5mut)-mGreenLantern

    Plasmid

    #219661

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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