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pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90287

Purpose

BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90288

Purpose

BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

pU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(KTF)-HA no-pA

Plasmid

#82596

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

W2101F

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDS2-aurZ

Plasmid

#70151

Purpose

Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

aurZ

Expression

Yeast

Promoter

Tef

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pPK1E-2+t

Plasmid

#48148

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pPK1E-2

Plasmid

#48146

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pPK1E-1

Plasmid

#48145

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pSA40

Plasmid

#35114

Depositor

John McCusker

Article

Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

Insert

lys5 flanked by loxp

Use

Yeast replicating

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-F1-GTG-F2-GGT-F3-GAA

Plasmid

#30707

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GTG, F2 GGT, F3 GAA

Use

Mrna production

Tags

HA

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
PrbC5

Plasmid

#24787

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Mutation

Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pET21a.MJ1228

Plasmid

#11410

Depositor

Sung-Hou Kim

Article

Kim et al Protein Sci. 1997 Oct . 6(10):2268-70.

Insert

eukaryotic translation initiation factor

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
brr2-Q904E pRS313

Plasmid

#111413

Purpose

brr2-Q904E (endogenous promoter)

Depositor

Christine Guthrie

Article

Mayerle et al bioRxiv 2018. 354514

Insert

BRR2

Expression

Yeast

Mutation

Q904E aa mutation

Availability

Academic Institutions and Nonprofits only

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brr2-R1107A pRS313

Plasmid

#111415

Purpose

brr2-R1107A (endogenous promoter)

Depositor

Christine Guthrie

Article

Mayerle et al bioRxiv 2018. 354514

Insert

BRR2

Expression

Yeast

Mutation

R1107A aa mutation

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:MelH

Plasmid

#257765

Purpose

For expression of untagged MelH

Depositor

Wilfred van der Donk

Article

Chen et al Proc Natl Acad Sci U S A. 2026 Apr 7;123

Insert

MelH

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:DybH

Plasmid

#257761

Purpose

For expression of untagged DybH

Depositor

Wilfred van der Donk

Article

Chen et al Proc Natl Acad Sci U S A. 2026 Apr 7;123

Insert

DybH

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:ChsH

Plasmid

#257762

Purpose

For expression of untagged ChsH

Depositor

Wilfred van der Donk

Article

Chen et al Proc Natl Acad Sci U S A. 2026 Apr 7;123

Insert

ChsH

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:HymH

Plasmid

#257763

Purpose

For expression of untagged HymH

Depositor

Wilfred van der Donk

Article

Chen et al Proc Natl Acad Sci U S A. 2026 Apr 7;123

Insert

HymH

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pTarget_dnaA-PAmCherry2.1

Plasmid

#212605

Purpose

Contains the E. coli codon-optimised gene of the photoactivatable fluorescent protein PAmCherry2(M10L), together with a sgRNA for SpydCas9 targeting the E. coli dnaA gene.

Depositor

John van der Oost

Article

Olivi et al Nat Commun. 2025 Aug 21;16(1):7813. doi:

Insert

PAmCherry2.1

Use

CRISPR

Mutation

M10L

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GADT7

Plasmid

#248657

Purpose

Empty vector for generating yeast 2-hybrid GAL4 activation domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 activation domain, HA

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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