We narrowed to 7,762 results for: loxP

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pBB3-ScVip1-536-1107-K554A

Plasmid

#238329

Purpose

ScVip1-536-1107-K554A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A

Plasmid

#238318

Purpose

ScVip1-536-1107-R547A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R551A

Plasmid

#238320

Purpose

ScVip1-536-1107-R551A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R551A

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-E991A

Plasmid

#238321

Purpose

ScVip1-536-1107-E991A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E991A

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H651A

Plasmid

#238323

Purpose

ScVip1-536-1107-H651A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H651A

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA595 - pBA904 Puro-T2A-mCherry

Plasmid

#238171

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Publication

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mCherry

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ECS-dSpyCatcher003

Plasmid

#234963

Purpose

Cre-dependent ECS-facing destabilized SpyCatcher003 (facing the extracellular space)

Depositor

Hajime Hirase

Publication

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-dSC

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

None

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK12-KiD/Cyclin K

Plasmid

#231743

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Publication

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1082)

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a-PAF1CdeltaCDC73

Plasmid

#231746

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Publication

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CTR9 (CTR9 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)
WDR61 (SKIC8 Human)

Tags

3C-Strep(II)

Expression

Insect

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG-GST(N)-CDK12RS(104-380)

Plasmid

#231738

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Publication

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-GST-3C

Expression

Insect

Mutation

CDK12(104-380)

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDC73deltaKIM(delta290-324)

Plasmid

#231739

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Publication

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDC73(delta290-324)

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDC73-YY/AA(Y290A, Y293A)

Plasmid

#231740

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Publication

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDC73(Y290A, Y293A)

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMCB320 sgControl (ACOCB)

Plasmid

#228115

Purpose

Safe targeting sgRNA

Depositor

Laura Attardi

Publication

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Safe targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMCB320 sgControl (GEA)

Plasmid

#228144

Purpose

Safe targeting sgRNA

Depositor

Laura Attardi

Publication

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Safe targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-137

Plasmid

#235159

Purpose

Expresses EGFP and miR-137 primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Publication

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/pri-miR-137 (Mir137 Mouse)

Use

AAV

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-CAG_G-PTEN

Plasmid

#227438

Purpose

An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.

Depositor

Tal Laviv

Publication

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN sensor (Pten Rat)

Use

AAV

Tags

CD-sREACh and mEGFP

Expression

Mammalian

Mutation

R14G

Promoter

pCAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHAND1-AM-Tag-PGK-Neo

Plasmid

#223216

Purpose

Targeting plasmid for inserting a C-terminal AM-Tag in the human HAND1 gene

Depositor

Matthew Birket

Publication

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Cre/Lox; Human targeting

Tags

AM-Tag

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLVTHM-Chim-Pchi-3xFLAG

Plasmid

#231536

Purpose

Expresses Salpingoeca helianthica chimeric Pchi in mammalian cells, for protein overexpression

Depositor

Ralf Jauch

Publication

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG flanked with N- and C-termini of mSox2

Tags

3xFLAG

Expression

Mammalian

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLVTHM-Chim-Salhel-I-3xFLAG

Plasmid

#231535

Purpose

Expresses Salpingoeca helianthica chimeric Sox-I in mammalian cells, for protein overexpression

Depositor

Ralf Jauch

Publication

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Salhel-I HMG flanked with N- and C-termini of mSox2

Tags

3xFLAG

Expression

Mammalian

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pWPI-Glis3M412

Plasmid

#216854

Purpose

This plasmid is for the production of lentiviral vectors expressing an active form of Glis3 together with GFP.

Depositor

Patrick Salmon

Insert

Glis3 (Glis3 Mouse)

Use

Lentiviral

Mutation

N-terminal deletion removing repressor domains

Promoter

EF1 alpha

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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