We narrowed to 14,023 results for: egf

1. pCMV-T7-ABE8e-SpCas9(MRKCRS)-P2A-EGFP (BKS1034)

   Plasmid

   #223117

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MRKCRS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MRKCRS)=D1135M, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

   Plasmid

   #234724

   Purpose

   All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

   Depositor

   Kirill Martemyanov

   Article

   Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   T2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSynI-EGFP-WPRE-hGH poly(A) (Alex_02)

   Plasmid

   #71651

   Purpose

   AAV expression of EGFP in human and mouse neurons

   Depositor

   Mirjana Maletić-Savatić

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

   Plasmid

   #232936

   Purpose

   Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   T2A-eGFP-SV40pA with Podxl homology arms
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX4T1_GST-EGFP-ATG13_IDR_191-517aa_(M196-H214 11xA)

   Plasmid

   #223779

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   ATG13 IDR (ATG13 Human)
   

   Use

   Tags

   GST-EGFP

   Expression

   Bacterial

   Mutation

   expresses aa 191-517, with M196A S197A R199A G202…

   Promoter

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX4T1_GST-EGFP-ATG13_IDR_delta206-230 (191-205_231-517aa)

   Plasmid

   #223787

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   ATG13 IDR (ATG13 Human)
   

   Use

   Tags

   GST-EGFP

   Expression

   Bacterial

   Mutation

   expresses aa 191-517, with 206-230 deleted

   Promoter

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa)

   Plasmid

   #223758

   Purpose

   Stable expression and inducible recruitment of protein in mammalian cell culture

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   WIPI2 IDR (WIPI2 Human)
   

   Use

   Lentiviral

   Tags

   FKBP-mEGFP

   Expression

   Mutation

   aa 364-425 only

   Promoter

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

   Plasmid

   #223724

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   FIP200-CTR (RB1CC1 Human)
   

   Use

   Tags

   6xHis-TEV-EGFP

   Expression

   Bacterial

   Mutation

   aa 1429-1591 only

   Promoter

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

   Plasmid

   #231367

   Purpose

   Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9

    Plasmid

    #231907

    Purpose

    For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9

    Plasmid

    #231909

    Purpose

    For packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-IRES-Luc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME nls EGFP WPRE bGH pA (JDW 488)

    Plasmid

    #224540

    Purpose

    A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-EGFP-WPRE-bGH-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)

    Plasmid

    #200283

    Purpose

    Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoter

    Depositor

    Kristina Ganzinger

    Article

    Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

    Insert

    SNAPtag-eGFP-CD86tm-eGFP-FKBP(f36v)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP

    Plasmid

    #223253

    Purpose

    AAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.

    Depositor

    Michael Do

    Article

    Liu et al Science. 2023 Jan 27;379(6630):376-381.

    Insert

    macaque OPN4-IRES-EGFP (OPN4 )
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin 1 (hSyn)

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)

    Plasmid

    #199582

    Purpose

    Expresses Chronos-eGFP in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Chronos eGFP
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a intron A

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312)

    Plasmid

    #225435

    Purpose

    BeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2.

    Depositor

    Diego Orzaez

    Insert

    LIR1:35S:eGFP:T35S-SIR:LIR2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

    Plasmid

    #221447

    Purpose

    Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    SPR degron: hGEM (1-110) fragment that is fused t…

    Promoter

    CMV

    Available Since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

    Plasmid

    #195018

    Purpose

    An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

    Depositor

    Christopher Richie

    Article

    Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

    Inserts

    CCGTATAGGCGGCTGCCCAA
    EGFP-KASH
    TTCCGTGGTGGGCAACGTAG
    

    Use

    AAV

    Tags

    KASH domain of Nesprin2

    Expression

    Mutation

    Promoter

    EF1a, hU6, and mU6

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNES-SET-EMD-EGFP (C-NES-SET-EMD)

    Plasmid

    #217770

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    SET (SET Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EMD domain (aa 70-226) plus nuclear export sequen…

    Promoter

    CMV

    Available Since

    June 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

    Plasmid

    #217763

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

    Promoter

    CMV

    Available Since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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