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pEMS2393

Plasmid

#246092

Purpose

For preclinical gene therapy: ss-AAV expression of N-term FLAG-tagged human PAX6 under control of Ple331 (PAX6 MiniPromoter); contains WPRE.

Depositor

Elizabeth Simpson

Article

Djaksigulova et al Gene Ther. 2026 Mar 9. doi: 10.1038/s414

Insert

ss-AAV-Ple331-3XFLAG/PAX6-WPRE (PAX6 Human)

Use

AAV

Tags

3XFLAG

Expression

Mammalian

Promoter

Ple311

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_AlphaCARD_ALFA_231232

Plasmid

#250443

Purpose

Expresses tagged alpha cardiac muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Alpha Cardiac Muscle Actin (ACTC1 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_GammaActin_ALFA_229230

Plasmid

#250440

Purpose

Expresses tagged gamma cytoplasmic actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T229/A230.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Gamma Cytoplasmic Actin (ACTG1 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2977-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank1_PC3 DM_AT_sg-213-219])

Plasmid

#251109

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2978-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank2_PC3 DM_AT_sg-234-240])

Plasmid

#251110

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2982-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank7_PC3 DM_AT_sg-241-247])

Plasmid

#251112

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124 D364E-His

Plasmid

#246711

Purpose

Expression vector for secreted human GPR124 ECD D364E (RGD -> RGE), N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD D364E

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-GPR124 LRR-His

Plasmid

#246695

Purpose

Expression vector for secreted human GPR124 LRR domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

LRR domain

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-GPR124 GAIN-His

Plasmid

#246694

Purpose

Expression vector for secreted human GPR124 GAIN domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

GAIN domain

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-spatzle-HA

Plasmid

#240235

Purpose

Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

spatzle (spz Fly)

Tags

HA-spGFP11

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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Bm iPGM-HiBiT

Plasmid

#218483

Purpose

Bacterially expressed iPGM-HiBiT for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Bm-iPGM-C-HiBIT-Thrombin-6XHis

Tags

HiBiT Tag and His-tag with thrombin site

Expression

Bacterial

Promoter

T7

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_TLE4:474-773

Plasmid

#210929

Purpose

Insect expression of human TLE4 fragment I474 to Y773. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TLE4:I474-Y773 (TLE4 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_LRRK2:2141-2527

Plasmid

#210899

Purpose

Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LRRK2:T2141-E2527 (LRRK2 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

M2397T natural variant ( VAR_024965)

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_BTRC:1-605

Plasmid

#211101

Purpose

Mammalian expression of full-length human BTRC. a N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

BTRC:M1-R605 (BTRC Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DDB1:1-1140

Plasmid

#211147

Purpose

Mammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDB1:M1-H1140 (DDB1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

loop deletion I396-D689

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_EIF3I:1-325

Plasmid

#211206

Purpose

Mammalian expression of full-length human EIF3I. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF3I:M1-A325 (EIF3I Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF12:1-453

Plasmid

#211198

Purpose

Mammalian expression of full-length human DCAF12 N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DNAI1:1-699

Plasmid

#211201

Purpose

Mammalian expression of full-length human DNAI1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DNAI1:M1-T699 (DNAI1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

A8S Y202C P495Q annotated sequence conflicts

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_DDB1:1-1140

Plasmid

#211188

Purpose

Mammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDB1:M1-H1140 (DDB1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

loop deletion I396-D689

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_CHAF1B:1-559

Plasmid

#211185

Purpose

Mammalian expression of full-length human CHAF1B. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CHAF1B:M1-P559 (CHAF1B Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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