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pEMS2113

Plasmid

#49138

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-emGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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AAVS1-CMV-V5-ZSWIM8

Plasmid

#254997

Purpose

Used to overexpress V5 tagged human ZSWIM8

Depositor

Joshua Mendell

Article

Han et al Science. 2020 Dec 18;370(6523). pii: sci

Insert

V5-ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

NA

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1-CAG-GCaMP6s

Plasmid

#241023

Purpose

An HDR plasmid for the AAVS1 safe harbor locus carrying a gene cassette consisting of GCaMP6s under control of a CAG promoter and a WPRE.

Depositor

Thomas Johnson

Article

Aguzzi et al Sci Transl Med. 2026 Apr 29;18(847):eadr

Insert

GCaMP6s and WPRE

Use

CRISPR

Tags

6x His Tag

Promoter

CAG

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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hAAVS-RPL23a-SNAP

Plasmid

#242187

Purpose

Express RPL23a-SNAP in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

RPL23a (RPL23A Human)

Tags

SNAP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_T11_EGFP-Responder

Plasmid

#230051

Purpose

It presents the T11 inducible promoter allowing the dox-dependent inducible activation of EGFP through the OPTi-OX platform

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

T11_EGFP

Expression

Mammalian

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_TRE3VG_mCherry-Responder

Plasmid

#230052

Purpose

It presents the TRE3VG inducible promoter allowing the dox-dependent inducible activation of mCherry through the OPTi-OX platform

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

mCherry

Expression

Mammalian

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_TRE3VG_EGFP-Responder

Plasmid

#230050

Purpose

It presents the TRE3VG inducible promoter allowing the dox-dependent inducible activation of EGFP through the OPTi-OX platform

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

TRE3VG_EGFP

Expression

Mammalian

Promoter

GAAGACAATAGCAGGCATG

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A+sgAAVS1_145

Plasmid

#213166

Purpose

Vector with sgAAVS1 for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgAAVS1_145

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A+sgAAVS1_118

Plasmid

#213167

Purpose

Vector with sgAAVS1 for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgAAVS1_118

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pNM220_AAVS1 integration helper

Plasmid

#211864

Purpose

Cas9 cutting at AAVS1 safe harbor locus

Depositor

Iain Cheeseman

Insert

AAVS1

Use

CRISPR

Mutation

N/A

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 SNAP-Sec61B

Plasmid

#207580

Purpose

Homologous recombination donor to integrate and expression cassette for SNAPtag-Sec61B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible SNAPTag-Sec61B

Tags

SNAPTAG

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Hb9-CD14

Plasmid

#204344

Purpose

Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.

Depositor

Ivo Lieberam

Article

Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

Insert

human CD14 (CD14 Human)

Expression

Mammalian

Promoter

mouse Hb9 (Mnx1) 9kb promoter fragment

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODALvar

Plasmid

#115640

Purpose

For targeted integration and inducible expression of a human NODAL splice variant using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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2ERT2-AAVS1 TALEN-L

Plasmid

#120544

Purpose

Drug inducible TALEN targeting the human AAVS1 locus for genome editing

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

ERT2, hAAVS1 1L TALEN

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

EF1a-short; U6

Available Since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
AV0-hSyn-SV40pA

Plasmid

#124965

Purpose

Express gene of interest under human Syn promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

AAV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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psubCMV-mVEGF-D-WPRE

Plasmid

#119226

Purpose

Plasmid encodes full-length (unprocessed) murine VEGF-D to be used for stimulation of lymphangiogenesis in mice in vivo.

Depositor

Kari Alitalo

Insert

Vegfd (Vegfd Mouse)

Use

AAV

Promoter

CMV

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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AICSDP-166:CDH1-mEGFP

Plasmid

#193921

Purpose

Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1

Depositor

Allen Institute for Cell Science

Insert

cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)

Use

AAV and CRISPR; Donor template

Tags

linker-mEGFP

Mutation

homology arms contain point mutations to disrupt …

Available Since

Dec. 22, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

Plasmid

#186993

Purpose

AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122, non-targeting crRNA guide

Use

AAV

Mutation

s1006-gggs-d1221 deletion

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (mPCSK9)

Plasmid

#170122

Purpose

AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (Pcsk9 Mouse)

Use

AAV

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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