We narrowed to 7,557 results for: aav

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pyc111f

Plasmid

#158780

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6f-RPL10a

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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IL1RN gRNA4

Plasmid

#113133

Purpose

Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, Synthetic Biology, and TALEN

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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IL1RN gRNA2

Plasmid

#113131

Purpose

Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_DIO_mMeCP2-HaloTag

Plasmid

#164062

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoter

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pyc111m

Plasmid

#158779

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m-RPL10a

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD_NES

Plasmid

#124706

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD_NES

Plasmid

#124630

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with MCP_ADAR1 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-U6sgbbBsmbI-puro-2A-Fluc

Plasmid

#100277

Purpose

Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1α:flex-mcherry

Plasmid

#222618

Purpose

Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

Depositor

Franck Polleux

Article

Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

Insert

Cre-dependent mCherry

Use

AAV

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_889

Plasmid

#216080

Purpose

ITRs from AAV2, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

ITRs from AAV2

Use

AAV; Destination

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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RNF130 shRNA-TRE

Plasmid

#225336

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 shRNA (Rnf130 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

TRE (tetracycline-responsive element)

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNAs-Crym

Plasmid

#200067

Purpose

For the knock down of Crym with an astrocyte specific mCherry expression

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

U6-3xsgRNA-Crym

Use

AAV, CRISPR, and Mouse Targeting

Tags

GfaABC1D-mCherry

Promoter

U6, GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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SpeCas9-HF

Plasmid

#192141

Purpose

Expresses highly specific SpeCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

SpeCas9-HF

Use

AAV

Expression

Mammalian

Mutation

SpeCas9-HF (R247A, N415A, S421A)

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-3xHA-SAPAP3

Plasmid

#190200

Purpose

Expresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

3xHA-SAPAP3 (Dlgap3 Mouse)

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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CSAC-Pers

Plasmid

#168281

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_DIO_R106W-HaloTag

Plasmid

#164063

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R106W mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 R106W (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCsy3-VPR-Csy4

Plasmid

#153943

Purpose

Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Insert

Csy3-VPR, Csy4

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCsy1-Csy2

Plasmid

#153942

Purpose

Expresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Insert

Csy1, Csy2

Use

AAV and CRISPR

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_DIO_R133C-HaloTag

Plasmid

#164064

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R133C mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 R133C (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2131

Plasmid

#111895

Purpose

ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Type

Empty backbone

Use

AAV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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