Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,877 results for: GAN

Showing: 7281 - 7300 of 7877 results

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AiP15022 - pAAV-AiE1398m-minBG-SYFP2-P2A-3XFLAG-H2B-WPRE3-BGHpA

Plasmid

#243314

Purpose

AiE1398m is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2-P2A-3XFLAG-H2B

Use

AAV

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Plasmid

#187077

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in the mitochondria matrix

Depositor

Kai Johnsson

Publication

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_LamP1- HaloTag7

Plasmid

#187078

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in lysosomes

Depositor

Kai Johnsson

Publication

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_LamP1- HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG-Floxed-FlpO-2A-H2BV5c.o.,rev.-WPRE

Plasmid

#234894

Purpose

CAG-driven, Cre-dependent FlpO recombinase for H2B-V5

Depositor

Silvia Arber

Publication

Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

Insert

Floxed-FlpO-2A-H2BV5

Use

AAV

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-LOV2-OMP25

Plasmid

#233346

Purpose

Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

Depositor

Noboru Mizushima

Publication

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N538E and N-terminus is deleted

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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aTY50_pPGK1_Pcad_IRES_PuroR_WPRE

Plasmid

#225348

Purpose

Lentiviral vector for Pcad expression

Depositor

Wendell Lim

Publication

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Pcad (Cdh3 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD 16S rRNA-2

Plasmid

#210834

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Publication

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast

Expression

Plant

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD 16S rRNA-1

Plasmid

#210833

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Publication

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast

Expression

Plant

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSN667

Plasmid

#219963

Purpose

Expressed sfGFP fused human KIF1Bß(1-721) in insect cells

Depositor

Shinsuke Niwa

Insert

codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)

Tags

sfGFP::2xStrepII tag

Expression

Insect

Mutation

encodes 1-721

Promoter

polyhedrin

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_WDR55:1-383

Plasmid

#210956

Purpose

Mammalian expression of full-length human WDR55. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR55:M1-D383 (WDR55 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

C151R natural variant (VAR_037057)

Promoter

CMV

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_EIF2A:1-427

Plasmid

#210939

Purpose

Insect expression of human EIF2A fragment M1 to T427. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF2A:M1-T427 (EIF2A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_IFT172:1-320

Plasmid

#210934

Purpose

Insect expression of human IFT172 fragment M1 to S320. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

IFT172:M1-S320 (IFT172 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TRAF7:382-670

Plasmid

#210930

Purpose

Insect expression of human TRAF7 fragment P382 to C670. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TRAF7:P382-C670 (TRAF7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_CORO7:1-925

Plasmid

#210921

Purpose

Insect expression of full-length human CORO7. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO7:M1-D925 (CORO7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_ATG16L2:321-619

Plasmid

#210918

Purpose

Insect expression of human ATG16L2 fragment A321 to Q619. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ATG16L2:A321-Q619 (ATG16L2 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_UTP4:1-686

Plasmid

#210915

Purpose

Insect expression of full-length human UTP4. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

UTP4:M1-R686 (CIRH1A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_DCAF13:54-356

Plasmid

#210908

Purpose

Insect expression of human DCAF13 fragment F54 to A356. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF13:F54-A356 (DCAF13 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

A86S sequence conflict

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR12:90-417

Plasmid

#210903

Purpose

Insect expression of human WDR12 fragment T90 to T417. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR12:T90-T417 (WDR12 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

introduced mutations F99A K131A R319S

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_RACK1:1-317

Plasmid

#210890

Purpose

Insect expression of full-length human RACK1. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RACK1:M1-R317 (RACK1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SMU1:170-513

Plasmid

#210882

Purpose

Insect expression of human SMU1 fragment K170 to P513. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SMU1:K170-P513 (SMU1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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