We narrowed to 59,357 results for: cloning

1. LentiV2-hU6-evopreQ1

   Plasmid

   #210189

   Purpose

   pegRNA/ngRNA library cloning

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mouse Clock delta 19

   Plasmid

   #177312

   Purpose

   expression of mutant mouse clock

   Depositor

   Joseph Takahashi

   Article

   King et al Cell. 1997 May 16;89(4):641-53. doi: 10.

   Insert

   mouse clock delta 19
   

   Expression

   Mammalian

   Mutation

   deletion from original mClock gene (514-564aa)

   Promoter

   CMV

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFLAG_attP

   Plasmid

   #110095

   Purpose

   Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3xFLAG

   Expression

   Bacterial

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-B/c

   Plasmid

   #227962

   Purpose

   Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   B/c
   

   Promoter

   None

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQTEV-PSMD11

   Plasmid

   #31333

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti_sgRNA(MS2)_zsGreen1

   Plasmid

   #138463

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   zsGreen1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO GFP HSPH1

   Plasmid

   #19480

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPH1 (HSPH1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET YBX-1

   Plasmid

   #13035

   Depositor

   Peter Newburger

   Article

   Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

   Insert

   YBX1 (YBX1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5/FRT/TO GFP HSPA1A

   Plasmid

   #19483

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPA1A (HSPA1A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPT-TAR C1

    Plasmid

    #141246

    Purpose

    Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Algal Research, 2020. 50, 102014

    Insert

    Mitochondrial Genome of Phaeodactylum tricornutum
    

    Use

    Synthetic Biology; Diatom expression

    Expression

    Bacterial and Yeast

    Mutation

    Minor additional mutations described in manuscript

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO V5 DNAJB2a

    Plasmid

    #19523

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2a (DNAJB2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRV2-GG

    Plasmid

    #105349

    Purpose

    virus-induced gene silencing

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    TRV-RNA2(5')
    

    Use

    In planta gene silencing; viral

    Mutation

    BsaI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Human Integrin beta 2

    Plasmid

    #8640

    Depositor

    Timothy Springer

    Article

    Kishimoto et al Cell 1987 Feb 27;48(4):681-90.

    Insert

    Integrin beta 2 (ITGB2 Human)
    

    Expression

    Mammalian

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pMC234r-ccdB/TPK2

    Plasmid

    #176179

    Purpose

    yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    dropout cassette containing toxic genes ccdB and TPK2
    

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiV2-mU6-evopreQ1

    Plasmid

    #210190

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUDP004

    Plasmid

    #101165

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187922

    Purpose

    Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase acidic protein
    

    Tags

    3xFLAG

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187918

    Purpose

    Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 1
    

    Tags

    3xFLAG

    Promoter

    CMV Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO V5 DNAJB2b

    Plasmid

    #19524

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2b (DNAJB2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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