Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 53 results for: cloning

Showing: 1 - 20 of 53 results

  1. FX cloning Kit

    Plasmid Kit
    #1000000039
    Purpose
    Insert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systems
    Depositor
    Eric Geertsma
    Available since
    April 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way
    Depositor
    Joachim Wittbrodt
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pMVP and pMAGIC Cloning Systems

    Plasmid Kit
    #1000000155
    Purpose
    pMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.
    Depositor
    Christopher Newgard
    Available since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  5. RAS mutant clone collection

    Plasmid Kit
    #1000000089
    Purpose
    Wild-type and mutant HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as Gateway entry clones
    Depositor
    Dominic Esposito
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. FNL Combinatorial Cloning Platform

    Plasmid Kit
    #1000000211
    Purpose
    A collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systems
    Depositor
    Dominic Esposito
    Available since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  7. 4G-Cloning Plasmid Kit

    Plasmid Kit
    #1000000252
    Purpose
    The 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.
    Depositor
    Stephan Gruber
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only

  8. Joint Modular Cloning (JMC) Toolkit

    Plasmid Kit
    #1000000233
    Purpose
    The Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional parts for assembling plant transformation vectors.
    Depositor
    Daniel Voytas
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only

  9. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit
    #1000000138
    Purpose
    Modular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expression
    Depositor
    Elena Caro Bernat
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid Kit
    #1000000191
    Purpose
    Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.
    Depositor
    Arnold Driessen
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only

  11. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid Kit
    #1000000156
    Purpose
    The MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.
    Depositor
    Scott Lenaghan
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only

  12. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9
    Depositor
    Irina Borodina
    Available since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. The Expanded EasyClone-MarkerFree toolkit

    Plasmid Kit
    #1000000190
    Purpose
    The Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.
    Depositor
    Irina Borodina
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  14. EasyClone 2.0 Yeast Toolkit

    Plasmid Kit
    #1000000073
    Purpose
    Integrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strains
    Depositor
    Irina Borodina
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. EasyCloneYALI- Integrative Vector Set

    Plasmid Kit
    #1000000140
    Purpose
    Contains integrative plasmids suitable for (over-)expression of (heterologous) genes in Y. lipolytica strains
    Depositor
    Irina Borodina
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. EasyCloneYALI- CRISPR/Cas9 Set

    Plasmid Kit
    #1000000141
    Purpose
    Contains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9
    Depositor
    Irina Borodina
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  17. RAS Pathway 2.0 Clone Collection

    Plasmid Kit
    #1000000070
    Purpose
    Collection contains 180 genes in the “RAS pathway version 2.0”, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning Platform
    Depositor
    Dominic Esposito
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. MultiSite Gateway plasmid toolKit

    Plasmid Kit
    #1000000050
    Purpose
    Cloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modules
    Depositor
    Tomas Santalucia
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. MoClo Plant Parts II and Infrastructure Kit

    Plasmid Kit
    #1000000135
    Purpose
    Compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs, with new phytobricks and vectors to connect Modular Cloning and Gateway Cloning platforms.
    Depositor
    Johannes Stuttmann
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. GreenGate

    Plasmid Kit
    #1000000036
    Purpose
    Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette
    Depositor
    Jan Lohmann
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 53 results