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We narrowed to 97 results for: cloning

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Showing: 1 - 20 of 97 results

  1. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

    Plasmid Kit
    #1000000171
    Purpose
    MoClo-compatible vectors for assembly of CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants.
    Depositor
    Sylvestre Marillonnet, Johannes Stuttmann
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Plant Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  2. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid Kit
    #1000000024
    Purpose
    Assemble TALEN constructs with custom repeat arrays between 12 and 31 repeats via Golden Gate cloning for expression in multiple systems.
    Depositor
    Adam Bogdanove, Daniel Voytas
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Mammalian Expression, Plant Expression, Yeast Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    Jan. 8, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    Cloning and assembly of DNA constructs for chromosomal integration in E. coli.
    Depositor
    Drew Endy
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Clonetegration
    Available Since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. GoldenBraid 2.0 Kit

    Plasmid Kit
    #1000000076
    Purpose
    Plant synthetic biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts.
    Depositor
    Diego Orzaez
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Plant Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (GoldenBraid)
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. MoClo Yeast Toolkit

    Plasmid Kit
    #1000000061
    Purpose
    Collection of standardized parts for hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression.
    Depositor
    John Dueber
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. MoClo Tool Kit

    Plasmid Kit
    #1000000044
    Purpose
    Collection of standardized genetic modules that can be used for hierarchical assembly of multi-gene expression constructs.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector Type
    Eukaryotic Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    April 8, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit
    #1000000060
    Purpose
    A plasmid toolbox used to construct vectors for constitutive or inducible gene expression, knockdown, deletion or editing.
    Depositor
    Ian Frew
    Application
    Cloning and Synthetic Biology
    Vector Type
    Lentiviral, Mammalian Expression
    Cloning Type
    MultiSite Gateway
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. GreenGate

    Plasmid Kit
    #1000000036
    Purpose
    Plasmids for rapidly assembling plant transformation constructs, based on the Golden Gate method.
    Depositor
    Jan Lohmann
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (GreenGate)
    Available Since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. S. cerevisiae Advanced Gateway Destination Vectors

    Plasmid Kit
    #1000000011
    Purpose
    Destination vectors for tagging and expression of genes in S. cerevisiae.
    Depositor
    Susan Lindquist
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    Gateway
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. Platinum Gate TALEN Kit

    Plasmid Kit
    #1000000043
    Purpose
    Construct Platinum TALEN vectors, with variable TALE repeats and non-RVD variations for expression in cultured cells, nematodes, sea urchins, ascidians, newts, frogs, mice, or rats.
    Depositor
    Takashi Yamamoto
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Eukaryotic Expression, Mammalian Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. CIDAR MoClo Parts Kit

    Plasmid Kit
    #1000000059
    Purpose
    Collection of standardized parts for combinatorial design and expression tuning in E. coli.
    Depositor
    Douglas Densmore
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. EcoFlex MoClo Kit

    Plasmid Kit
    #1000000080
    Purpose
    Collection of DNA parts for synthetic biology use in E. coli, cell-free protein synthesis, protein purification, and hierarchical assembly of transcription units.
    Depositor
    Paul Freemont
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. FX cloning Kit

    Plasmid Kit
    #1000000039
    Purpose
    Use fragment exchange (FX) cloning to generate constructs for screening protein expression in several organisms with a variety of N- and C-terminal tags.
    Depositor
    Eric Geertsma
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression, Xenopus Expression, Yeast Expression
    Cloning Type
    FX Cloning
    Available Since
    April 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Plasmids for introduction of genes into 11 predetermined yeast chromosomal loci using CRISPR/Cas9.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit
    #1000000030
    Purpose
    Supplemental kit for #1000000024 includes modified pFUS vectors to simplify Golden Gate assembly and mammalian expression/mRNA synthesis vectors containing unique shorter N- and C-terminal domains.
    Depositor
    Takashi Yamamoto
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    In Vitro Transcription, Mammalian Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    March 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. MoClo CRISPR/Cas Toolkit for Plants

    Plasmid Kit
    #1000000159
    Purpose
    Plasmids for gene editing using Cas nucleases and base editors in monocots and dicots.
    Depositor
    Vladimir Nekrasov
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Plant Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. LIC TAL Effector Assembly Kit

    Plasmid Kit
    #1000000023
    Purpose
    A TALE repeat unit library of 2-mer fragments that can be assembled via Ligation-independent cloning (LIC) into mammalian expression-ready TALEN constructs in 2 hierarchical assembly steps.
    Depositor
    Veit Hornung
    Application
    Genome Editing
    Vector Type
    Mammalian Expression
    Editing Type
    TALEN
    Available Since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    A collection that combines Golden Gate with Multisite Gateway cloning for assembly of complex fusion proteins or transgenesis constructs.
    Depositor
    Joachim Wittbrodt
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression, Eukaryotic Expression, Mammalian Expression, Zebrafish Expression
    Cloning Type
    Golden Gate
    Available Since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. Multiplex Yeast Toolkit (MYT)

    Plasmid Kit
    #1000000229
    Purpose
    Collection of plasmids which extend the capabilities of the MoClo Yeast Toolkit (YTK) for genetically engineering S. cerevisiae.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Nov. 9, 2023
    Availability
    Academic Institutions and Nonprofits only

  20. MoClo Pichia toolkit

    Plasmid Kit
    #1000000108
    Purpose
    A set of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit (#1000000061) for protein expression and secretion in Pichia pastoris.
    Depositor
    Volker Sieber
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 97 results