We narrowed to 59,184 results for: cloning

1. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

   Plasmid

   #55218

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6

   Plasmid

   #192184

   Purpose

   Clone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 6 heavy chain (HH06)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2

   Plasmid

   #192183

   Purpose

   Clone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 2 heavy chain (HH02)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Clone 16 - 3 HK02 pFUSE-IgG-human kappa VL-CH1 hole-clone2

   Plasmid

   #192185

   Purpose

   Clone 16 (HK02) pFUSE-IgG-human kappa VL-CH1 hole-clone2

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 2 light chain (HK02)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

   Plasmid

   #55219

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   His6

   Expression

   Insect

   Available Since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

   Plasmid

   #55220

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   His6 and MBP, Asn10

   Expression

   Insect

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

   Plasmid

   #55221

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   StrepII and msfGFP

   Expression

   Insect

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

   Plasmid

   #55222

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

   Plasmid

   #55223

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Available Since

   July 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPIP_Peptide_Cloning_Vector

    Plasmid

    #172110

    Purpose

    Lentiviral plasmid for protein / peptide overexpression

    Depositor

    Prashant Mali

    Article

    Ford et al Cell Syst. 2021 Jul 21;12(7):716-732.e7.

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    Ef1-alpha

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA_CloningVector_tagBFP

    Plasmid

    #163747

    Purpose

    Empty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. gRNA_CloningVector_miRFP670

    Plasmid

    #163748

    Purpose

    Empty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. XClone_Cas9_KRAB_T2A_BFP

    Plasmid

    #141208

    Purpose

    Tunable and temporal expression control of dCas9

    Depositor

    Rory Johnson

    Insert

    dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. psglessClonePX

    Plasmid

    #87193

    Purpose

    used for downstream cloning, contains CMV promoter with NheI and XhoI cloning sites flanking a partial insert that gets excised when adding an insert of choice

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA_Cloning Vector

    Plasmid

    #41824

    Purpose

    An empty gRNA expression vector, used to create a gRNA to a specific sequence.

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    AflII site for Gibson Assembly

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCC1-4k-BBclone2

    Plasmid

    #137070

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0323
    P13
    DipA
    Lmp1
    

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pErCas12a EZ Clone

    Plasmid

    #132641

    Purpose

    Expresses ErCas12a and an sgRNA to target a region of interest

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    AAV and CRISPR

    Tags

    3x HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q926R (Please see depositor comments) and BsaI si…

    Promoter

    CMV Promoter

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDL52 (clone45)

    Plasmid

    #188578

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPEPZ-sgRNAclone

    Plasmid

    #141090

    Purpose

    This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

    Insert

    mCherry
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    p3

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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