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Plasmid#192172Purposeexpress SARS2-NSP1-truncDepositorInsertexpress SARS2-NSP1-trunc (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI
Plasmid#192113PurposeExpresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-BA.4/5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SARS-CoV-Spike[WIV1 RBD]
Plasmid#190087Purposeexpress chimeric SARS-CoV spike with the WIV-1 receptor binding domainDepositorInsertSARS-CoV S (S Synthetic)
UseTagsRhodopsin C9ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SARS-CoV-Spike[Khosta2 RBD]
Plasmid#190083Purposeexpress chimeric SARS-CoV spike with the Khosta-2 receptor binding domainDepositorInsertSARS-CoV S (S Synthetic)
UseTagsRhodopsin C9ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SARS-CoV-Spike[Khosta1 RBD]
Plasmid#190082Purposeexpress chimeric SARS-CoV spike with the Khosta-1 receptor binding domainDepositorInsertSARS-CoV S (S Synthetic)
UseTagsRhodopsin C9ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SARS-CoV-Spike[BtKY72 RBD]
Plasmid#190081Purposeexpress chimeric SARS-CoV spike with the BtKY72 receptor binding domainDepositorInsertSARS-CoV S (S Synthetic)
UseTagsRhodopsin C9ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SARS-CoV-Spike[PRD0038 RBD]
Plasmid#190085Purposeexpress chimeric SARS-CoV spike with the PRD0038 receptor binding domainDepositorInsertSARS-CoV S (S Synthetic)
UseTagsRhodopsin C9ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-BA.2-AVI
Plasmid#184531PurposeExpresses SARS-CoV-2 S2P protein from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-BA.2 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24-, P25-, P26-, A27S, G142D, V213G, G339D…PromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-C.37-AVI
Plasmid#181699PurposeExpresses SARS-CoV-2 RBD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-C.37 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationL452Q, F490SPromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-C.37-AVI
Plasmid#181696PurposeExpresses SARS-CoV-2 NTD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-C.37 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationG75V, T76I, R246N, ∆S247-∆D253,PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-C.37-AVI
Plasmid#181693PurposeExpresses SARS-CoV-2 S2P protein from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-C.37 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationG75V, T76I, R246N, ∆S247-∆D253, L452Q, F490S, D61…PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-AY.1-AVI
Plasmid#176437PurposeExpresses SARS-CoV-2 NTD domain from the AY.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-AY.1 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19R, T95I, G142D, del156-157, R158G, W258LPromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-AY.1-AVI
Plasmid#176335PurposeExpresses SARS-CoV-2 S2P protein from the AY.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-AY.1 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19R, T95I, G142D, del156-157, R158G, W258L, K417…PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCell-Free_KA1_SARS-COV-2_S-frag3
Plasmid#169403PurposeCell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFPDepositorInsertN-term frag. S protein after the furin cleavag (N SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)
Plasmid#169162PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cellsDepositorInsertnsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
UseTags6His-3xFlagExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)
Plasmid#169163PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cellsDepositorInsert3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
UseTags3xFlag-6HisExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK_P-GAP-LM_lacO-cP-AOX1-sAR
Plasmid#126745Purposeyeast plasmid overexpressing sLovA,CPR and LacI-Mit1ADDepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterGAPAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-ICL1-LM_lacO-cP-AOX1-sAR
Plasmid#126744Purposeyeast plasmid overexpressing sLovA,CPR and LacI-Mit1ADDepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterICL1Available sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FFSARG-ddFLN4-HIS-ybbr
Plasmid#101240Purposeminimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFFSARG peptide, ddFLN4 fingerprint
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V/D91G/V220A
Plasmid#47647PurposepAC-EsaR with a208g, a272g, c659t mutations in esaRDepositorInsertesaR-I70V/D91G/V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Isoleucine 70 to Valine, Aspartic acid 91…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only