...sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS classic) sequences....entry of SARS-CoV and SARS-CoV-2 viruses. ( 1 ) TMPRSS2 - a serine protease that primes the SARS-CoV-...novel virus. It is now known that SARS-CoV-2 entry, like that of SARS-CoV and MERS CoV, depends on binding...cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV is hACE2, an angiotensin receptor. Another...this page: SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing SARS-CoV-2 sequences...COVID-19 research. Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences are now available...and packaging plasmids. SARS-CoV-2 Lentiviral preps now available for several SARS-CoV-2 constructs from...

...pseudotyping with SARS-CoV-2 spike protein.... Collections COVID-19 - Viral Pseudotyping SARS-CoV-2 Pseudotyped...Pseudotyped Virus Mechanisms of SARS-CoV-2 infection remain poorly understood but are critical for developing...viral spread. As a Biosafety Level 3 (BSL-3) agent, SARS-CoV-2 requires specialized facilities for the study...as replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface...cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single round of viral entry and infection...of viral particles have been pseudotyped with the SARS-CoV-2 Spike protein and you will find examples of...

...Plasmids SARS-CoV-2 Plasmids The following types of plasmids are available: Bacterial expression: SARS-CoV-...Browse plasmids encoding SARS-CoV-2 viral proteins. Depositor...experiments. In an effort to speed up research of SARS-CoV-2, Ginkgo Bioworks is using their DNA design...build DNA that will enable researchers to express SARS-CoV-2 viral proteins in several expression systems...expression and/or purification Yeast expression: SARS-CoV-2 genes in yeast expression vectors with various...expression and/or purification Mammalian expression: SARS-CoV-2 genes in mammalian expression vectors with...protein expression and/or purification Truncated SARS-CoV-2 Spike gene constructs for making VSV pseudovirus...

...Description PI 141391 pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro Expression of SARS-CoV-2 nucleocapsid (N) protein...Krogan 141386 pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro Expression of SARS-CoV-2 membrane (M) protein...Krogan 141385 pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro Expression of SARS-CoV-2 envelope (E) protein...Krogan 141383 pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro Expression of SARS-CoV-2 orf3a protein Krogan...Krogan 141347 pBOB-CAG-SARS-CoV2-Spike-HA Expression of SARS-CoV-2 spike (S) protein Pao 154985 pWPI-IRES-Puro-Ak-ACE2...

... for SARS-CoV-2 spike pseudotyping, visit the SARS-CoV-2 Pseudotyped Virus collection page. SARS-CoV-2...reaction to detect SARS-CoV-2 nucleic acids. Mammalian targets During the 2003 SARS outbreak, Hyeryun ...functional receptor for SARS-CoV. The lab also found that cathepsins play a role in SARS-CoV infection and ...inhibitors of SARS-CoV-2 entry and become upregulated during viral infection.  Expression of SARS-CoV-2 viral...reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image from ...labs. Spike protein, variants, and pseudotyping The SARS-CoV-2 virus depends on its spike protein to bind...and reagents for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited the plasmids...

...developed during the SARS-CoV pandemic more than a decade ago are now being used to study SARS-CoV-2. The resurgence...replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-CoV with implications for clinical manifestations... glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun 11... need fast access to the tools required to study SARS-CoV-2, but with many academic research labs closed... as biobanks, are crucial for the advancement of SARS-CoV-2 research because they provide rapid access...Cell lines Many of the reagents used to investigate SARS-CoV-2 were developed for broad use and are now important...susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional cell line...

...and MTA Information Featured Collections COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins...

...pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 N protein  pLVX-EF1alpha-SARS-CoV-2...the SARS-CoV-2 M protein pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro: Expression of the SARS-CoV-...lentiviral preps SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins...protein (pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro...last few months: Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs...

... 1: Illustration of SARS-CoV-2 from Philippa Steinberg for the IGI.   What SARS-CoV-2 plasmids from...Ginkgo Bioworks to synthesize and share over 1,850 SARS-CoV-2 plasmids to help academic and industrial labs... for request? Currently, plasmids containing all SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae...bacterial expression systems, Ginkgo synthesized SARS-CoV-2 expression plasmids in a high copy backbone...for the high level expression and purification of SARS-CoV-2 proteins for downstream experiments and offer...optimized, full length and truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven...Ginkgo’s first deposited set of plasmids containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae...

... coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants...several months, we’ve seen the emergence of several SARS-CoV-2 variants coincide with increased vaccination...patients. They were able to tweak this assay to study SARS-CoV-2 spike variants. “We created a series of spike...vaccine. This sera was used in the high-throughput SARS-CoV-2 pseudovirus neutralization assay. Neutralization...on the effectiveness of current vaccines against SARS-CoV-2 variants, this study was the first to compare...Lessells RJ, de Oliveira T, Sigal A (2021) Escape of SARS-CoV-2 501Y.V2 from neutralization by convalescent...Iafrate AJ, Naranbhai V, Balazs AB (2021) Multiple SARS-CoV-2 variants escape neutralization by vaccine-...

...comprehensive SARS-CoV-2 virus-human interaction network in which we expressed 26 of the 29 SARS-CoV-2 viral...since we all became acutely aware of the threat of SARS-CoV-2. Like many in the science community, we’ve...lifecycle, identifying these potential human helpers of SARS-CoV-2 (especially the ones that are druggable) is...We are just at the beginning of understanding how SARS-CoV-2 interacts with human proteins. For example...scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected in various ways...key details. Our set of constructs comprises all SARS-CoV-2 viral proteins except for Nsp3 and Nsp16, ...out what is going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct is undergoing...

...from Iowa State University. Since its appearance, SARS-CoV-2 has spread to almost every part of the world....   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity... by primers that target the E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the ... similar method has been optimized to detect the SARS-CoV-2 virus directly using saliva without having...ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter of RNA...single-molecule sensitive. In the case of HIV-1 and SARS-CoV-2 detection, AIOD-CRISPR without preamplification...easy-to-deploy, protocol for Cas13-based detection of SARS-CoV-2 genetic material (CREST) Sensitivity: 10 copies...

... Benjie Chen. Last week, the Addgene 10-year Anniversary Gala celebrated all of the people who have helped...this lovely play-by-play of Addgene's 10 Year Anniversary Gala. The Addgene lab often works as a very well-oiled... addgene's 10 year anniversary...

...post: Pooled library to study antibody escape in SARS-CoV-2 Recombinant antibodies for aminoacyl tRNA ...-to-use viral preps Mapping antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As...severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus may acquire mutations to escape... the viral spike protein.   Figure 1. (A) SARS-CoV-2 RBD DMS Library generation and sequencing....display library. From Starr et al Science., 2021. SARS-CoV-2 infects host cells through binding the human...responsible for binding ACE2 is the target of most anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes...protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD is displayed on the yeast cell’s...

...reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We also highlighted...plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our hope that by providing...working with RNA and extracting RNA without a kit. 3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR...detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids deposited at...

...more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein into ...role in infecting cell lines without using intact SARS-CoV-2 virus. Scientists have been using similar ...2018).   Figure 2: To pseudotype with the SARS-CoV-2 spike protein, scientists encode the spike...Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays. Viruses...

...also offer export license applications for VSV and SARS-COV-1 plasmids. Export control license process  ... is charged.  Timelines  We have offered VSV and SARS-COV-1 plasmids under this application process since...

...pandemic, qPCR can be used to diagnose the presence of SARS-CoV-2 RNA, but inadequate access to reagents and...has shared a protocol for using SHERLOCK to detect SARS-CoV-2 RNA. The test is started using RNA purified... recently adapted the DETECTR platform to detect SARS-CoV-2 using a lateral flow strip format. They published..., Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038...

...rapidly identify and isolate sybodies targeting the SARS-CoV-2 Receptor Binding Domain found on the membrane-bound...Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2 sybodies! References Geertsma ER (2013) ...et al. (2020) Synthetic nanobodies targeting the SARS-CoV-2 receptor-binding domain. BioRxiv.  https:/...

...constructs for tagged and untagged expression of SARS-CoV-2 proteins in bacteria, yeast, and mammalian...synthesized approximately 3,000 constructs to support SARS-CoV-2 research.  Browse the entire Ginkgo Bioworks...