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We narrowed to 274 results for: sars

Showing: 1 - 20 of 274 results

  1. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... for SARS-CoV-2 spike pseudotyping, visit the SARS-CoV-2 Pseudotyped Virus collection page. SARS-CoV-2...reaction to detect SARS-CoV-2 nucleic acids. Mammalian targets During the 2003 SARS outbreak, Hyeryun ...functional receptor for SARS-CoV. The lab also found that cathepsins play a role in SARS-CoV infection and ...inhibitors of SARS-CoV-2 entry and become upregulated during viral infection.  Expression of SARS-CoV-2 viral...reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image from ...labs. Spike protein, variants, and pseudotyping The SARS-CoV-2 virus depends on its spike protein to bind...and reagents for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited the plasmids...

  2. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...developed during the SARS-CoV pandemic more than a decade ago are now being used to study SARS-CoV-2. The resurgence...replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-CoV with implications for clinical manifestations... glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun 11... need fast access to the tools required to study SARS-CoV-2, but with many academic research labs closed... as biobanks, are crucial for the advancement of SARS-CoV-2 research because they provide rapid access...Cell lines Many of the reagents used to investigate SARS-CoV-2 were developed for broad use and are now important...susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional cell line...

  3. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 N protein  pLVX-EF1alpha-SARS-CoV-2...the SARS-CoV-2 M protein pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro: Expression of the SARS-CoV-...lentiviral preps SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins...protein (pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro...last few months: Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs...

  4. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ... 1: Illustration of SARS-CoV-2 from Philippa Steinberg for the IGI.   What SARS-CoV-2 plasmids from...Ginkgo Bioworks to synthesize and share over 1,850 SARS-CoV-2 plasmids to help academic and industrial labs... for request? Currently, plasmids containing all SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae...bacterial expression systems, Ginkgo synthesized SARS-CoV-2 expression plasmids in a high copy backbone...for the high level expression and purification of SARS-CoV-2 proteins for downstream experiments and offer...optimized, full length and truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven...Ginkgo’s first deposited set of plasmids containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae...

  5. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    Published
    June 22, 2021, 1:15 p.m.
    ... coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants...several months, we’ve seen the emergence of several SARS-CoV-2 variants coincide with increased vaccination...patients. They were able to tweak this assay to study SARS-CoV-2 spike variants. “We created a series of spike...vaccine. This sera was used in the high-throughput SARS-CoV-2 pseudovirus neutralization assay. Neutralization...on the effectiveness of current vaccines against SARS-CoV-2 variants, this study was the first to compare...Lessells RJ, de Oliveira T, Sigal A (2021) Escape of SARS-CoV-2 501Y.V2 from neutralization by convalescent...Iafrate AJ, Naranbhai V, Balazs AB (2021) Multiple SARS-CoV-2 variants escape neutralization by vaccine-...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...from Iowa State University. Since its appearance, SARS-CoV-2 has spread to almost every part of the world....   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity... by primers that target the E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the ... similar method has been optimized to detect the SARS-CoV-2 virus directly using saliva without having...ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter of RNA...single-molecule sensitive. In the case of HIV-1 and SARS-CoV-2 detection, AIOD-CRISPR without preamplification...easy-to-deploy, protocol for Cas13-based detection of SARS-CoV-2 genetic material (CREST) Sensitivity: 10 copies...

  7. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...comprehensive SARS-CoV-2 virus-human interaction network in which we expressed 26 of the 29 SARS-CoV-2 viral...since we all became acutely aware of the threat of SARS-CoV-2. Like many in the science community, we’ve...lifecycle, identifying these potential human helpers of SARS-CoV-2 (especially the ones that are druggable) is...We are just at the beginning of understanding how SARS-CoV-2 interacts with human proteins. For example...scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected in various ways...key details. Our set of constructs comprises all SARS-CoV-2 viral proteins except for Nsp3 and Nsp16, ...out what is going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct is undergoing...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...Addgene repository of tools for SAR-CoV-2 research. Two new antibodies, Anti-SARS-CoV-2 Nucleocapsid Protein...mBG17] and Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86], target distinct epitopes on the SARS-CoV-2 nucelocapsid.... Find Anti-SARS-CoV-2 Antibodies here! Terry, J.S., et al. (2021). Development of a SARS-CoV-2 nucleocapsid...Improved prime editor PE7 Antibodies to facilitate SARS-CoV-2 research New viral vector preps of Voltron2...07259-6. PMID: 38570691. Antibodies to facilitate SARS-CoV-2 research   By Ashley Waldron Ready-to-use ...    Figure 3: HeLa cells transduced with SARS-CoV-2 nucleocapsid protein (Plasmid #141391-LV [...negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies #211756 (left...

  9. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...This year marks Addgene's 20th anniversary! We've been celebrating throughout the year, and on Sunday...Sunday, June 9th, we hosted our 20th anniversary party for all Addgenies to celebrate together. We were even...

  10. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...post: Pooled library to study antibody escape in SARS-CoV-2 Recombinant antibodies for aminoacyl tRNA ...-to-use viral preps Mapping antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As...severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus may acquire mutations to escape... the viral spike protein.   Figure 1. (A) SARS-CoV-2 RBD DMS Library generation and sequencing....display library. From Starr et al Science., 2021. SARS-CoV-2 infects host cells through binding the human...responsible for binding ACE2 is the target of most anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes...protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD is displayed on the yeast cell’s...

  11. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... Benjie Chen. Last week, the Addgene 10-year Anniversary Gala celebrated all of the people who have helped...this lovely play-by-play of Addgene's 10 Year Anniversary Gala. The Addgene lab often works as a very well-oiled... addgene's 10 year anniversary...

  12. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We also highlighted...plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our hope that by providing...working with RNA and extracting RNA without a kit. 3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR...detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids deposited at...

  13. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein into ...role in infecting cell lines without using intact SARS-CoV-2 virus. Scientists have been using similar ...2018).   Figure 2: To pseudotype with the SARS-CoV-2 spike protein, scientists encode the spike...Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays. Viruses...

  14. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...also offer export license applications for VSV and SARS-COV-1 plasmids. Export control license process  ... is charged.  Timelines  We have offered VSV and SARS-COV-1 plasmids under this application process since...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...pandemic, qPCR can be used to diagnose the presence of SARS-CoV-2 RNA, but inadequate access to reagents and...has shared a protocol for using SHERLOCK to detect SARS-CoV-2 RNA. The test is started using RNA purified... recently adapted the DETECTR platform to detect SARS-CoV-2 using a lateral flow strip format. They published..., Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038...

  16. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...rapidly identify and isolate sybodies targeting the SARS-CoV-2 Receptor Binding Domain found on the membrane-bound...Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2 sybodies! References Geertsma ER (2013) ...et al. (2020) Synthetic nanobodies targeting the SARS-CoV-2 receptor-binding domain. BioRxiv.  https:/...

  18. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ..., Addgene gave to me: five methods for detecting SARS-CoV-2 using CRISPR.  On the sixth day of CRISPR,...

  20. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...to develop that this illness was caused by either SARS-CoV-2, Adenovirus type 41 (AdV-41), or Adenovirus-Associated...unusual cases was either 1) a physiological effect of SARS-CoV-2 toward destabilizing one’s natural immune ...: 10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular...
Showing: 1 - 20 of 274 results