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Addgene
Showing: 1 - 20 of 28 results
  1. COVID-19 Resources

    Type
    Collection
    ...sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS classic) sequences....entry of SARS-CoV and SARS-CoV-2 viruses. ( 1 ) TMPRSS2 - a serine protease that primes the SARS-CoV-...novel virus. It is now known that SARS-CoV-2 entry, like that of SARS-CoV and MERS CoV, depends on binding...cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV is hACE2, an angiotensin receptor. Another...this page: SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing SARS-CoV-2 sequences...COVID-19 research. Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences are now available...and packaging plasmids. SARS-CoV-2 Lentiviral preps now available for several SARS-CoV-2 constructs from...
  2. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...pseudotyping with SARS-CoV-2 spike protein.... Collections COVID-19 - Viral Pseudotyping SARS-CoV-2 Pseudotyped...Pseudotyped Virus Mechanisms of SARS-CoV-2 infection remain poorly understood but are critical for developing...viral spread. As a Biosafety Level 3 (BSL-3) agent, SARS-CoV-2 requires specialized facilities for the study...as replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface...cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single round of viral entry and infection...of viral particles have been pseudotyped with the SARS-CoV-2 Spike protein and you will find examples of...
  3. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...Plasmids SARS-CoV-2 Plasmids The following types of plasmids are available: Bacterial expression: SARS-CoV-...Browse plasmids encoding SARS-CoV-2 viral proteins. Depositor...experiments. In an effort to speed up research of SARS-CoV-2, Ginkgo Bioworks is using their DNA design...build DNA that will enable researchers to express SARS-CoV-2 viral proteins in several expression systems...expression and/or purification Yeast expression: SARS-CoV-2 genes in yeast expression vectors with various...expression and/or purification Mammalian expression: SARS-CoV-2 genes in mammalian expression vectors with...protein expression and/or purification Truncated SARS-CoV-2 Spike gene constructs for making VSV pseudovirus...
  4. Lentiviral Prep Service

    Type
    Collection
    ...Description PI 141391 pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro Expression of SARS-CoV-2 nucleocapsid (N) protein...Krogan 141386 pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro Expression of SARS-CoV-2 membrane (M) protein...Krogan 141385 pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro Expression of SARS-CoV-2 envelope (E) protein...Krogan 141383 pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro Expression of SARS-CoV-2 orf3a protein Krogan...Krogan 141347 pBOB-CAG-SARS-CoV2-Spike-HA Expression of SARS-CoV-2 spike (S) protein Pao 154985 pWPI-IRES-Puro-Ak-ACE2...
  5. Distribution to Industry

    Type
    Collection
    ...and MTA Information Featured Collections COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins...
  6. Bacterial Expression Systems

    Type
    Collection
    ...promoter repressed by EsaR. Designed to be used with the pAC-EsaR Series . pAC-EsaR Series Various Plasmids...transcription factors, and reporter genes. pCS-PesaRlux 47640 PesaR 3OC6HSL (Quorum sensing molecule) Cynthia...inducible expression of EsaR, a transcription factor responsive to 3OC6HSL. EsaR derivatives found herein...sensitivities to 3OC6HSL. The EsaR-responsive promoter can be found in pCS-PesaRlux controlling luciferase expression...
  7. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...skeletal type Sarcomeric thin filaments 114412 TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres (M-line tag) ...mEGFP MLC-2a Sarcomeric thick filaments 114414 MYL2-mEGFP AICSDP-49 mEGFP MLC-2v Sarcomeric thick filaments...Cytoplasm 114405 ATP2A2-mEGFP AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP...124607 ACTN2-mEGFP AICSDP-63 mEGFP Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T...
  8. CRISPR Guide

    Type
    Collection
    ...Results Resources Web References PAM Sequences Glossary Publications CRISPR Overview Class 2 C lustered...synthetic RNA composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20 nucleotide...First, consider the genetic manipulation that is necessary to model your specific disease or process of interest...specific application, and in such cases, it may be necessary to customize an existing reagent to suit your ...cells Packaging and envelope plasmids provide the necessary components to make lentiviral particles (for details...ideally less than 10 bp away. In this case, it is necessary to identify the exact location where the edit ...-target activity A PAM sequence is absolutely necessary for Cas9 to bind target DNA. As such, one can ...
  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ...member 2 BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR NR1I3 nuclear receptor subfamily 1, group I,...CD32B, FCG2, FCGR2, IGFR2 FOS v-fos FBJ murine osteosarcoma viral oncogene homolog AP-1, C-FOS GSK3B glycogen...synthase kinase 3 beta - HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1...oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog C-K-RAS, K-RAS2A, K-RAS2B...3, LIR3, MGC138403, PIRB LYN v-yes-1 Yamaguchi sarcoma viral related oncogene homolog FLJ26625, JTK8 MALT1...platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) FLJ12858, PDGF2... death. Symbol Name Synonyms ARAF v-raf murine sarcoma 3611 viral oncogene homolog A-RAF, ARAF1, PKS2,...
  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...membrane voltage with improved brightness (QuasAr1 and QuasAr2) All-optical electrophysiology in mammalian...in C. elegans (Arch(D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based voltage imaging tools...stress on cells. Nat Commun. 2021 Sep 14;12(1):5438. Cesar Cuevas-Velazquez , Jose Dinneny Lysosomal pH Lyso-pHluorin...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters...
  11. AAVED

    Type
    Collection
    ... Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan Janelia Research Campus Kenton Woodard...10:15 AM AAV Retrograde Tracing Gowan Tervo and Sarada Viswanathan 11:00 AM Circuit Tracing Ali Cetin ..., Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research Campus Kenton Woodard...
  12. Arf GTPase Family

    Type
    Collection
    ...10139 201 Gateway GTPase Sar1a 56681 198 Bacterial (pET), Gateway GTPase Sar1b 51128 198 Gateway GTPase...
  13. Ras Pathway

    Type
    Collection
    ...Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral oncogene homolog GRB2 Growth factor receptor...exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma viral oncogene homolog Muscle RAS oncogene homolog...
  14. Lentivirus Plasmids

    Type
    Collection
    ...Frequently asked questions about lentivirus use Glossary of commonly used lentiviral terms Popular Lentiviral...lentivirus used in research is that the components necessary to produce an infectious viral particle (a virion...
  15. Neuroscience

    Type
    Collection
    ...the responsibility of the authors and does not necessarily represent the official views of the National ...
  16. All Antibodies

    Type
    Collection
    ...the responsibility of the authors and does not necessarily represent the official views of the National ...
  17. Adenovirus Plasmids

    Type
    Collection
    ...construction Frequently asked questions about adenovirus Glossary of commonly used adenovirus terms Adenoviral Strains...
  18. Qi Lab CRISPR Page

    Type
    Collection
    ...MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA...
Showing: 1 - 20 of 28 results