We narrowed to 13,668 results for: GAN

1. pRSET-Cm-CspA1

   Plasmid

   #215398

   Purpose

   Cm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)

   Depositor

   Gitta Coaker

   Article

   Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

   Insert

   cspA1
   

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli BL21(DE3)

   Promoter

   T7 promoter

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2_-87prox1a:basEGFP_Acry:GFP

   Plasmid

   #218213

   Purpose

   Zebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lens

   Depositor

   Katarzyna Koltowska

   Article

   Panara et al Development. 2024 May 1;151(9):dev202525

   Insert

   -87prox1a
   

   Use

   Zebrafish expression

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pssAAV_Seq17_CMV_bglobin_3NLS_TFP_WPRE_polyA

   Plasmid

   #220223

   Purpose

   AAV-STARR-seq validation control vector with TFP

   Depositor

   Simon Rumpel

   Article

   Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM-AAV-hIsl2-EGFP

   Plasmid

   #153206

   Purpose

   Human Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRhaCAST

   Plasmid

   #211791

   Purpose

   pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter

   Depositor

   Silvia Cardona

   Article

   Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Rhamnose-inducible

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mFzd4CRDL(42-203)-6xHis_pAcGP67a

   Plasmid

   #216379

   Purpose

   Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   6xHis and GP64 signal sequence

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human USP47

   Plasmid

   #217624

   Purpose

   Mammalian expression of USP47

   Depositor

   Ethan Lee

   Article

   Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

   Insert

   USP47 (USP47 Human)
   

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. NFAST(low)-Halo-ER

   Plasmid

   #214419

   Purpose

   ER localization of low-affinity NFAST fused to Halo-ER

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspA-N
   

   Tags

   Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG NC

   Plasmid

   #214675

   Purpose

   Negative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tags

   Depositor

   Eugene Yeo

   Article

   Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   FLAG
   

   Tags

   MCP and V5

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT_IFL_A28C_R

    Plasmid

    #200184

    Purpose

    WT PA14 I-F Leader and A28C Repeat

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Wildtype I-F Leader and a mutant I-F Locus (A28C)
    

    Expression

    Bacterial

    Mutation

    A28C in the I-F repeat

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR2_IRdist_scram

    Plasmid

    #162319

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    The distal Upstream Motif site is scrambled

    Promoter

    pBAD

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid

    #202587

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE60 G121A wgMDH

    Plasmid

    #204252

    Purpose

    Bacterial expression of G121A wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G121A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE60 R196E wgMDH

    Plasmid

    #204272

    Purpose

    Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196E

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE60 P123A wgMDH

    Plasmid

    #204254

    Purpose

    Bacterial expression of P123A wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    P123A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE60 R130Q wgMDH

    Plasmid

    #204268

    Purpose

    Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130Q

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE60 G127F wgMDH

    Plasmid

    #204264

    Purpose

    Bacterial expression of G127F wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G127F

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE60 P126R wgMDH

    Plasmid

    #204261

    Purpose

    Bacterial expression of P126R wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    P126R

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE60 G127A wgMDH

    Plasmid

    #204263

    Purpose

    Bacterial expression of G127A wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G127A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQE60 M66L wgMDH

    Plasmid

    #204247

    Purpose

    Bacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    M66L

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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