Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 15,748 results for: pET

Showing: 8141 - 8160 of 15748 results

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(loxPcon-DIAL-YB_TATA)-mGL-BGH_pKG2787

Plasmid

#246327

Purpose

loxPcon DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-psfGFPcp-natMX6

Plasmid

#240557

Purpose

Tag S. pombe genes at 3' end (C-terminal end of protein) with circularly permuted superfolder GFP, codon-optimized for S. pombe

Depositor

Silke Hauf

Article

Kamenz et al Mol Cell. 2015 Nov 5;60(3):446-59. doi:

Insert

psfGFPcp

Expression

Yeast

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-aptamer

Plasmid

#236373

Purpose

crRNA array expression

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

Aptamer array

Use

CRISPR and Lentiviral

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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U6-miR.FF4

Plasmid

#235256

Purpose

Non-intronic (U6-driven) expression of miR-FF4 (FF4 hairpin)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FF4 hairpin

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E3GFP

Plasmid

#233008

Purpose

Galactose iduced expression of Gcn4 E+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F124IHA

Plasmid

#232993

Purpose

Galactose iduced expression of Gcn4 F124IHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F124I

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

F124I

Promoter

Gal1

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_FL Gcn4

Plasmid

#232955

Purpose

Galactose iduced expression of full-length Gcn4 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120AHA

Plasmid

#232992

Purpose

Galactose iduced expression of Gcn4 W120AHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBDHA

Plasmid

#232987

Purpose

Galactose iduced expression of Gcn4 DBDAHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

DBD

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merHA

Plasmid

#232988

Purpose

Galactose iduced expression of Gcn4 WT 44merHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F124IGFP

Plasmid

#233005

Purpose

Galactose iduced expression of Gcn4 F124IGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F124I

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

F124I

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+HA

Plasmid

#232991

Purpose

Galactose iduced expression of Gcn4 WW+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 L123A

Plasmid

#232969

Purpose

Galactose iduced expression of Gcn4 L123A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L123A

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap2

Plasmid

#232965

Purpose

Galactose iduced expression of Gcn4 k0.19 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.19

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120AGFP

Plasmid

#233004

Purpose

Galactose iduced expression of Gcn4 120AGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 K5

Plasmid

#232966

Purpose

Galactose iduced expression of Gcn4 K+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 K+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103K, D115K, D127K, D133K, E143K

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap3

Plasmid

#232960

Purpose

Galactose iduced expression of Gcn4 k0.47 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R2

Plasmid

#232970

Purpose

Galactose iduced expression of Gcn4 R+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R+

Tags

TEV cleavage site

Expression

Yeast

Mutation

K118R, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E+

Plasmid

#232963

Purpose

Galactose iduced expression of Gcn4 E+in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120A

Plasmid

#232962

Purpose

Galactose iduced expression of Gcn4 W120A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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