We narrowed to 25,635 results for: pET

1. HotPETase_pET21b

   Plasmid

   #215407

   Purpose

   Expression construct for HotPETase gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   HotPETase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21b(+)-Is-PETase

   Plasmid

   #112202

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
   

   Use

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET24a isPETase

   Plasmid

   #225759

   Purpose

   Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   isPETase
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21b FAST-PETase

   Plasmid

   #225760

   Purpose

   Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   FAST-PETase
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21b Hot-PETase

   Plasmid

   #225761

   Purpose

   Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   Hot-PETase
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-hIRF5-V5(4D)-LPETG

   Plasmid

   #237225

   Purpose

   Expresses LPETG-tagged, constitutively active human IRF5 variant in bacteria

   Depositor

   Elliot Chaikof

   Article

   Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

   Insert

   Interferon regulatory factor 5 (IRF5 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S451D, S453D, S456D, S462D

   Promoter

   Available Since

   July 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21b(+)-Is-PETase

   Plasmid

   #112202

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

   Depositor

   Gregg Beckham, Christopher Johnson

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
   

   Use

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-21b(+)-Is-PETase-W185A

   Plasmid

   #112204

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
   

   Use

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W185A

   Promoter

   N/A

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21b(+)-Is-PETase-W159H-S238F

   Plasmid

   #112203

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
   

   Use

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W159H, S238F

   Promoter

   N/A

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-Cre-LPETG-6xHis

    Plasmid

    #237224

    Purpose

    Expresses LPETG-tagged Cre recombinase in bacteria

    Depositor

    Elliot Chaikof

    Article

    Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

    Insert

    Cre-LPETG (cre Synthetic)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-Cys-ELP(40nm)-LPETGG

    Plasmid

    #90475

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    Cys-ELP(40nm)-LPETGG
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-Cys-ELP(120nm)-LPETGG

    Plasmid

    #90472

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    Cys-ELP(120nm)-LPETGG
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-CohIII-CBM-HIS-LPETGG

    Plasmid

    #91697

    Purpose

    CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    CohIII-CBM-HIS-LPETGG
    

    Use

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-21b(+)-Is-PETase-W185A

    Plasmid

    #112204

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W185A

    Promoter

    N/A

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid

    #112203

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W159H, S238F

    Promoter

    N/A

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-Cys-ELP(40nm)-LPETGG

    Plasmid

    #90475

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    Cys-ELP(40nm)-LPETGG
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-Cys-ELP(120nm)-LPETGG

    Plasmid

    #90472

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    Cys-ELP(120nm)-LPETGG
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-CohIII-CBM-HIS-LPETGG

    Plasmid

    #91697

    Purpose

    CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    CohIII-CBM-HIS-LPETGG
    

    Use

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

    Plasmid

    #40731

    Depositor

    Daniel Gerlich

    Article

    Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

    Insert

    Aurora B FRET-sensor
    

    Use

    Tags

    CyPet and YPet

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

    Plasmid

    #40571

    Depositor

    Daniel Gerlich

    Article

    Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

    Insert

    Aurora B FRET-sensor N-terminally fused to histone 2B
    

    Use

    Tags

    CyPet and YPet

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge