EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont PS
ACS Synth Biol. 2016 May 2. PubMed Article
Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont PS
ACS Synth Biol. 2016 May 2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 72934 | pTU1 | Backbone vector with internal BsaI removed by site-directed mutagenesis |
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| 72935 | pTU1-A-lacZ | Level 1 Destination vector - Position A |
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| 72936 | pTU1-B-lacZ | Level 1 Destination vector - Position B |
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| 72937 | pTU1-C-lacZ | Level 1 Destination vector - Position C |
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| 72938 | pTU1-D-lacZ | Level 1 Destination vector - Position D |
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| 72939 | pTU1-A-RFP | Level 1 Destination vector - Position A |
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| 72940 | pTU1-B-RFP | Level 1 Destination vector - Position B |
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| 72941 | pTU1-C-RFP | Level 1 Destination vector - Position C |
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| 72942 | pTU1-D-RFP | Level 1 Destination vector - Position D |
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| 72943 | pTU1-D1-RFP | Level 1 Destination vector - Position D1 |
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| 72944 | pTU1-E-RFP | Level 1 Destination vector - Position E |
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| 72945 | pTU3-A-RFP | Level 3 Destination vector - Assembly of 2 Level 2 modules - AB |
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| 72946 | pTU3-B-RFP | Level 3 Destination vector - Assembly of 4 Level 2 modules - ABCD |
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| 72947 | pBP | Backbone vector with internal BsmBI removed by site-directed mutagenesis |
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| 72948 | pBP-lacZ | Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning |
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| 72949 | pBP-ORF | Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site |
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| 72950 | pTU2-A-lacZ | Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
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| 72951 | pTU2-B-lacZ | Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
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| 72952 | pTU2-C-lacZ | Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
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| 72953 | pTU2-D-lacZ | Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
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| 72954 | pTU2-A-RFP | Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
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| 72955 | pTU2-B-RFP | Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
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| 72956 | pTU2-C-RFP | Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
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| 72957 | pTU2-D-RFP | Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
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| 72958 | pTU2-a-RFP | Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB |
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| 72959 | pTU2-b-RFP | Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC |
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| 72960 | pBP-ORF-eGFP | Level 0 Entry vector" ORF - eGFP " |
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| 72961 | pBP-ORF-eCFP | Level 0 Entry vector" ORF - eCFP" |
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| 72962 | pBP-ORF-mCherry | Level 0 Entry vector" ORF - mCherry " |
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| 72963 | pBP-J23100 | Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion) |
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| 72964 | pBP-J23108 | Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion) |
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| 72965 | pBP-J23114 | Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion) |
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| 72966 | pBP-SJM901 | Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion) |
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| 72967 | pBP-SJM902 | Level 0 - SJM902 promoter (5' CTAT/3' GTAC fusion) |
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| 72968 | pBP-SJM903 | Level 0 - SJM903 promoter (5' CTAT/3' GTAC fusion) |
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| 72969 | pBP-SJM905 | Level 0 - SJM905 promoter (5' CTAT/3' GTAC fusion) |
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| 72970 | pBP-SJM906 | Level 0 - SJM906 promoter (5' CTAT/3' GTAC fusion) |
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| 72971 | pBP-SJM908 | Level 0 - SJM908 promoter (5' CTAT/3' GTAC fusion) |
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| 72972 | pBP-SJM910 | Level 0 - SJM910 promoter (5' CTAT/3' GTAC fusion) |
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| 72973 | pBP-SJM911 | Level 0 - SJM911 promoter (5' CTAT/3' GTAC fusion) |
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| 72974 | pBP-SJM912 | Level 0 - SJM912 promoter (5' CTAT/3' GTAC fusion) |
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| 72975 | pBP-SJM914 | Level 0 - SJM914 promoter (5' CTAT/3' GTAC fusion) |
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| 72976 | pBP-SJM915 | Level 0 - SJM915 promoter (5' CTAT/3' GTAC fusion) |
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| 72977 | pBP-T7 | T7 promoter (5' CTAT/3' GTAC fusion) |
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| 72978 | pBP-T7_RBS | T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion) |
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| 72979 | pBP-T7_RBS-His6-Thrombin | T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion) |
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| 72980 | pBP_BBa_B0034 | Bba_B0034 iGEM RBS (5' GTAC/3' CATA fusion) |
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| 72981 | pBP-pET-RBS | PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion) |
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| 72982 | pBP-Tag_linker | N-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion) |
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| 72983 | pBP-HexHis | Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion) |
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| 72984 | pBP-Strep(II) | Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion) |
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| 72985 | pBP-TL1 | RBS_H1 |
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| 72986 | pBP-TL2 | RBS_H2 |
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| 72987 | pBP-TL3 | RBS_H3 |
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| 72988 | pBP-TL4 | RBS_H5 |
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| 72989 | pBP-TL5 | RBS_M2 |
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| 72990 | pBP-TL6 | RBS_M3 |
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| 72991 | pBP-TL7 | RBS_M5 |
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| 72992 | pBP-TL8 | RBS_L1 |
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| 72993 | pBP-TL9 | RBS_L2 |
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| 72994 | pBP-TL10 | RBS_L3 |
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| 72995 | pBP-TL11 | RBS_L4 |
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| 72996 | pBP-TL12 | RBS_L5 |
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| 72997 | pBP-BBa_B0012 | BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion) |
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| 72998 | pBP-BBa_B0015 | BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion) |
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| 72999 | pBP-L3S2P21 | L3S2P21 terminator |
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| 73000 | pBP-L3S1P51 | L3S1P51 terminator |
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| 73001 | pBP-L3S1P32 | L3S1P32 terminator |
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| 73002 | pBP-L3S1P11 | L3S1P11 terminator |
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| 73003 | pBP-L2U8H11 | L2U8H11 terminator |
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| 73004 | pBP-L2U5H11 | L2U5H11 terminator |
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| 73005 | pBP-L2U5H08 | L2U5H08 terminator |
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| 73006 | pBP-L2U3H03 | L2U3H03 terminator |
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| 73007 | pBP-L2U2H09 | L2U2H09 terminator |
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| 73008 | pBP-L1U1H09 | L1U1H09 terminator |
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| 73009 | pBP-L1U1H08 | L1U1H08 terminator |
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| 73010 | pTU2_2a | Secondary module (BpiI) site in pTU2a backbone |
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| 73011 | pTU2_2b | Secondary module (BpiI) site in pTU2b backbone |
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| 74088 | pTU2S-a (p15A origin) | Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication |
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| 74089 | pTU2S-b (p15A origin) | Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication |
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| 74090 | pTU2-A-RFP (p15A origin) | pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication |
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| 74091 | pTU2S-a (colE1 origin) | Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication |
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| 74092 | pTU2S-b (colE1 origin) | Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication |
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| 74093 | pTU2-A-RFP (colE1 origin) | pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication |
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| 74094 | pBP-Bba_K822000 | L-Lactate inducible promoter from E. coli MG1655 |
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| 74095 | pBP-lldR | L-lactate repressor (LldR) from E. coli MG1655 |
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| 74096 | pBP-T7RNAP | T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA |
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| 74097 | pBP-BBa_J23100 | BBa_J23100 promoter (iGEM collection) |
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| 74098 | pBP-TL9 | TL9 RBS (AAAAAG) |
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