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EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont PS
ACS Synth Biol. 2016 May 2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
72934pTU1Backbone vector with internal BsaI removed by site-directed mutagenesis
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72935pTU1-A-lacZLevel 1 Destination vector - Position A
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72936pTU1-B-lacZLevel 1 Destination vector - Position B
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72937pTU1-C-lacZLevel 1 Destination vector - Position C
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72938pTU1-D-lacZLevel 1 Destination vector - Position D
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72939pTU1-A-RFPLevel 1 Destination vector - Position A
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72940pTU1-B-RFPLevel 1 Destination vector - Position B
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72941pTU1-C-RFPLevel 1 Destination vector - Position C
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72942pTU1-D-RFPLevel 1 Destination vector - Position D
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72943pTU1-D1-RFPLevel 1 Destination vector - Position D1
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72944pTU1-E-RFPLevel 1 Destination vector - Position E
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72945pTU3-A-RFPLevel 3 Destination vector - Assembly of 2 Level 2 modules - AB
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72946pTU3-B-RFPLevel 3 Destination vector - Assembly of 4 Level 2 modules - ABCD
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72947pBPBackbone vector with internal BsmBI removed by site-directed mutagenesis
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72948pBP-lacZEntry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning
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72949pBP-ORFEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
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72950pTU2-A-lacZLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
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72951pTU2-B-lacZLevel 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
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72952pTU2-C-lacZLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
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72953pTU2-D-lacZLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
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72954pTU2-A-RFPLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
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72955pTU2-B-RFPLevel 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
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72956pTU2-C-RFPLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
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72957pTU2-D-RFPLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
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72958pTU2-a-RFPLevel 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB
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72959pTU2-b-RFPLevel 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC
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72960pBP-ORF-eGFPLevel 0 Entry vector" ORF - eGFP "
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72961pBP-ORF-eCFPLevel 0 Entry vector" ORF - eCFP"
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72962pBP-ORF-mCherryLevel 0 Entry vector" ORF - mCherry "
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72963pBP-J23100Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)
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72964pBP-J23108Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)
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72965pBP-J23114Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)
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72966pBP-SJM901Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)
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72967pBP-SJM902Level 0 - SJM902 promoter (5' CTAT/3' GTAC fusion)
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72968pBP-SJM903Level 0 - SJM903 promoter (5' CTAT/3' GTAC fusion)
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72969pBP-SJM905Level 0 - SJM905 promoter (5' CTAT/3' GTAC fusion)
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72970pBP-SJM906Level 0 - SJM906 promoter (5' CTAT/3' GTAC fusion)
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72971pBP-SJM908Level 0 - SJM908 promoter (5' CTAT/3' GTAC fusion)
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72972pBP-SJM910Level 0 - SJM910 promoter (5' CTAT/3' GTAC fusion)
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72973pBP-SJM911Level 0 - SJM911 promoter (5' CTAT/3' GTAC fusion)
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72974pBP-SJM912Level 0 - SJM912 promoter (5' CTAT/3' GTAC fusion)
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72975pBP-SJM914Level 0 - SJM914 promoter (5' CTAT/3' GTAC fusion)
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72976pBP-SJM915Level 0 - SJM915 promoter (5' CTAT/3' GTAC fusion)
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72977pBP-T7T7 promoter (5' CTAT/3' GTAC fusion)
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72978pBP-T7_RBST7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)
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72979pBP-T7_RBS-His6-ThrombinT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)
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72980pBP_BBa_B0034Bba_B0034 iGEM RBS (5' GTAC/3' CATA fusion)
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72981pBP-pET-RBSPET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)
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72982pBP-Tag_linkerN-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion)
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72983pBP-HexHisHexahistidine N-terminal tag (5' TAAA/3' CATA fusion)
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72984pBP-Strep(II)Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)
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72985pBP-TL1RBS_H1
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72986pBP-TL2 RBS_H2
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72987pBP-TL3RBS_H3
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72988pBP-TL4RBS_H5
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72989pBP-TL5RBS_M2
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72990pBP-TL6RBS_M3
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72991pBP-TL7RBS_M5
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72992pBP-TL8RBS_L1
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72993pBP-TL9RBS_L2
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72994pBP-TL10RBS_L3
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72995pBP-TL11RBS_L4
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72996pBP-TL12RBS_L5
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72997pBP-BBa_B0012BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)
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72998pBP-BBa_B0015BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)
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72999pBP-L3S2P21L3S2P21 terminator
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73000pBP-L3S1P51L3S1P51 terminator
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73001pBP-L3S1P32L3S1P32 terminator
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73002pBP-L3S1P11L3S1P11 terminator
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73003pBP-L2U8H11L2U8H11 terminator
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73004pBP-L2U5H11L2U5H11 terminator
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73005pBP-L2U5H08L2U5H08 terminator
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73006pBP-L2U3H03L2U3H03 terminator
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73007pBP-L2U2H09L2U2H09 terminator
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73008pBP-L1U1H09L1U1H09 terminator
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73009pBP-L1U1H08L1U1H08 terminator
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73010pTU2_2aSecondary module (BpiI) site in pTU2a backbone
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73011pTU2_2bSecondary module (BpiI) site in pTU2b backbone
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74088pTU2S-a (p15A origin)Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication
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74089pTU2S-b (p15A origin)Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication
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74090pTU2-A-RFP (p15A origin)pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication
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74091pTU2S-a (colE1 origin)Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
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74092pTU2S-b (colE1 origin)Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
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74093pTU2-A-RFP (colE1 origin)pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication
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74094pBP-Bba_K822000L-Lactate inducible promoter from E. coli MG1655
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74095pBP-lldRL-lactate repressor (LldR) from E. coli MG1655
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74096pBP-T7RNAPT7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA
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74097pBP-BBa_J23100BBa_J23100 promoter (iGEM collection)
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74098pBP-TL9TL9 RBS (AAAAAG)
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