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Joint Universal Modular Plasmids (JUMP): A flexible and comprehensive platform for synthetic biology
Valenzuela-Ortega M, French C
bioRxiv, October 10, 2019
Article

Joint Universal Modular Plasmids (JUMP) is a universal vector for Modular Cloning (Golden Gate) assembly. JUMP is a flexible platform based on Standard European Vector Architecture (SEVA), compatible with PhytoBricks and BioBricks (and the Registry of Standard Biological Parts). The toolkit includes vectors with four selection markers, ten origins of replication (integrative and episomal with diverse copy number), and a collection of microbial basic parts. To allow tailored modification, all vectors can be modified using modular cloning in an orthogonal way using multiple cloning sites. This allows researchers to introduce any feature into any JUMP vector and simplifies the Design-Build-Test cycle of synthetic biology.

Joint Universal Modular Plasmids

Please see the Supplementary Information in the bioRxiv preprint for detailed methodologies.

Plasmids from Article

ID Plasmid Purpose  
126956pJUMP19-min2"Minimal cargo" vector. Allows introducing any part using BsmBI; but doesn't have cloning reporter. Can be used to generate new promoter/terminator acceptor vectors.
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126957pJUMP19-PacPromoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.
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126958pJUMP19-TacTerminator acceptor vector. Minimal cargo vector; with eGFP in downstream secondary site and constitutive promoter J23100 in upstream site. Allows introducing any part using BsmBI.
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126959pJUMP18-UacUniversal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8.
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126960pJUMP29-1A(sfGFP)Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126961pJUMP29-1B(sfGFP)Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126962pJUMP29-1C(sfGFP)Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126963pJUMP29-1D(sfGFP)Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126964pJUMP49-2A(sfGFP)Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126965pJUMP49-2B(sfGFP)Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126966pJUMP49-2C(sfGFP)Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126967pJUMP49-2D(sfGFP)Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126968pJUMP21-1A(sfGFP)Level 1 vector. sfGFP reporter. Origin R6K (pir conditional).
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126969pJUMP22x-1A(sfGFP)Level 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.
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126970pJUMP23-1A(sfGFP)Level 1 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)
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126971pJUMP24-1A(sfGFP)Level 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
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126972pJUMP25-1A(sfGFP)Level 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)
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126973pJUMP26-1A(sfGFP)Level 1 vector. sfGFP reporter. Origin p15A (medium copy number)
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126974pJUMP27-1A(sfGFP)Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number)
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126975pJUMP27ts-1A(sfGFP)Level 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)
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126976pJUMP28-1A(sfGFP)Level 1 vector. sfGFP reporter. Origin pUC (high copy number)
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126977pJUMP27ts-1A(lacZ)Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number)
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126978pJUMP29-1A(lacZ)Level 1 vector. With lacZ as alternative cloning reporter. OriV 9 (pBBR322/ROP; medium copy number).
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126979pJUMP29[dCas9]-1A(lacZ)Level 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.
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126980pJUMP27ts[mCherry]-1A(lacZ)Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.
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126981pJUMP29-1D'(sfGFP)Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126982pJUMP29-1E(sfGFP)Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126983pJUMP39-1A(sfGFP)Level 1 vector with alternative selection marker (chloramphenicol). OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126984pJUMP38-1A(sfGFP)Level 1 vector with alternative selection marker (chloramphenicol). Origin pUC (high copy number). sfGFP cloning reporter.
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126985pJUMP41-2A(sfGFP)Level 2 vector. sfGFP reporter. Origin R6K (pir conditional).
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126986pJUMP42x-2A(sfGFP)Level 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.
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126987pJUMP43-2A(sfGFP)Level 2 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)
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126988pJUMP44-2A(sfGFP)Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
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126989pJUMP45-2A(sfGFP)Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)
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126990pJUMP46-2A(sfGFP)Level 2 vector. sfGFP reporter. Origin p15A (medium copy number)
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126991pJUMP47-2A(sfGFP)Level 2 vector. sfGFP reporter. Origin pSC101 (low copy number)
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126992pJUMP47ts-2A(sfGFP)Level 2 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)
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126993pJUMP48-2A(sfGFP)Level 2 vector. sfGFP reporter. Origin pUC (high copy number)
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126994pJUMP49-2D'(sfGFP)Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126995pJUMP49-2E(sfGFP)Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.
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126996pJUMP19-J23100_PBasic Part P- Promoter (constitutive); J23100
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126997pJUMP19-J23102_PBasic Part P- Promoter (constitutive); J23102.
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126998pJUMP19-J23103_PBasic Part P- Promoter (constitutive); J23103.
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126999pJUMP19-J23106_PBasic Part P- Promoter (constitutive); J23106.
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127000pJUMP19-J23107_PBasic Part P- Promoter (constitutive); J23107.
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127001pJUMP19-J23119s_PBasic Part P- Promoter (constitutive); J23119 (1bp short on 3').
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127002pJUMP19-T7pro_PBasic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymerase
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127003pJUMP19-Pbad_PBasic Part P- Promoter (non-constitutive); Pbad (I13453), arabinose induced; controlled by AraC.
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127004pJUMP19-Ptac_PBasic Part P- Promoter (non-constitutive); Ptac; IPTG/lactose induced; controlled by LacI.
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127005pJUMP19-Ptet_PBasic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetR
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127006pJUMP19-Pci_PBasic Part P- Promoter (non-constitutive); Pci; controlled by cI.
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127007pJUMP19-Pars_PBasic Part P- Promoter (non-constitutive); Pars; metal sensitive; controlled by arsR
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127008pJUMP19-Pmer_PBasic Part P- Promoter (non-constitutive); Pmer; metal sensitive; controlled by merR
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127009pJUMP19-Pcue_PBasic Part P- Promoter (non-constitutive); Pcue; metal sensitive; controlled by CueR
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127010pJUMP18-B0032_RBasic Part R- RBS; B0032m Ribosome Binding Site.
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127011pJUMP18-B0033_RBasic Part R- RBS; B0033m Ribosome Binding Site.
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127012pJUMP18-B0034_RBasic Part R- RBS; B0034m Ribosome Binding Site.
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127013pJUMP18-RBS-pET_RBasic Part R- RBS; pET vector Ribosome Binding Site.
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127014pJUMP18-RBS-pET-MV_RNBasic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start.
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127015pJUMP18-B0032-MV_RNBasic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + Met-Val N-terminus start.
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127016pJUMP18-B0033-MV_RNBasic Part RN- RBS+N-terminus; B0034m Ribosome Binding Site + Met-Val N-terminus start.
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127017pJUMP18-B0034-MV_RNBasic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start.
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127018pJUMP18-sfGFP_OBasic Part O- ORF; sfGFP; fluorescent protein
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127019pJUMP18-amilCP_OBasic Part O- ORF; amilCP; blue chromoprotein
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127020pJUMP18-araC_OBasic Part O- ORF; araC; transcription factor
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127021pJUMP18-tetR_OBasic Part O- ORF; TetR; transcription factor
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127022pJUMP18-arsR_OBasic Part O- ORF; arsR; transcription factor
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127023pJUMP18-merR_OBasic Part O- ORF; merR; transcription factor
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127024pJUMP18-lacI_OBasic Part O- ORF; lacI; transcription factor
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127025pJUMP18-mCherry_OBasic Part O- ORF; mCherry; fluorescent protein
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127026pJUMP18-cueR_OBasic Part O- ORF; cueR; transcription factor
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127027pJUMP18-lacZ_OBasic Part O- ORF; lacZ alpha-peptide; blue/white screening.
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127028pJUMP18-dCas9_OBasic Part O- ORF; Catalytically dead mutant of the Cas9 from Streptococcus pyogenes.
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127029pJUMP18-cI_OBasic Part O- ORF; cI; transcription factor from lambda phage.
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127030pJUMP18-cI-ts_OBasic Part O- ORF; cI; transcription factor from lambda phage; thermosensitive.
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127031pJUMP18-pheS_NOCBasic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.
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127032pJUMP18-lambdaRED_NOCBasic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).
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127033pJUMP18-Histag_NBasic Part N- N-terminus; polyhistidine tag
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127034pJUMP18-Streptag_NBasic Part N- N-terminus; Strep-tag II
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127035pJUMP18-Histag_CBasic Part C- C-terminus; polyhistidine tag
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127036pJUMP18-Streptag_CBasic Part C- C-terminus; Strep-tag II
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127037pJUMP19-L3S1P51_TBasic Part T- Terminator; L3S1P51; synthetic terminator
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127038pJUMP19-B0015_TBasic Part T- Terminator; B0015. Terminator.
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127039pJUMP19-L3S2P21_TBasic Part T- Terminator; L3S2P21; synthetic terminator
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127040pJUMP19-L3S1P32_TBasic Part T- Terminator; L3S1P32; synthetic terminator
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127041pJUMP19-L3S1P11_TBasic Part T- Terminator; L3S1P11; synthetic terminator
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127042pJUMP19-L2U2H09_TBasic Part T- Terminator; L2U2H09; synthetic terminator
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127043pJUMP19-L1U1H09_TBasic Part T- Terminator; L1U1H09; synthetic terminator
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127044pJUMP19-L1U1H08_TBasic Part T- Terminator; L1U1H08; synthetic terminator
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127045pJUMP19-B0015_CTBasic Part CT- C-terminus + Terminator; B0015. C-termini STOP + Terminator.
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127046pJUMP19-L3S2P21_CTBasic Part CT- C-terminus + Terminator; L3S2P21. C-termini STOP + terminator (synthetic)
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127047pJUMP19-L3S1P51_CTBasic Part CT- C-terminus + Terminator; L3S1P51. C-termini STOP + terminator (synthetic)
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127048pJUMP19-L2U2H09_CTBasic Part CT- C-terminus + Terminator; L2U2H09. C-termini STOP + terminator (synthetic)
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127049pJUMP19-L1U1H09_CTBasic Part CT- C-terminus + Terminator; L1U1H09. C-termini STOP + terminator (synthetic)
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127050pJUMP19-L1U1H08_CTBasic Part CT- C-terminus + Terminator; L1U1H08. C-termini STOP + terminator (synthetic)
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127051pJUMP18-cat_TUBasic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.
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