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We narrowed to 62,186 results for: cloning

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Showing: 8181 - 8200 of 62186 results

  2. pUS250

    Plasmid
    #198322
    Purpose
    Cumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoprotein
    Depositor
    Nicholas Coleman, Mark Somerville
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    cumate-inducible
    Available Since
    April 12, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    198322-plasmid-map-sequence-id-390064

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  3. pGEX6P1-DEST-Strep2

    Plasmid
    #119753
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119753-plasmid-map-sequence-id-243311

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  4. MZB117

    Plasmid
    #228481
    Purpose
    "Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    GST (glutathione S-transferase)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228481-plasmid-map-sequence-id-454022

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  5. Topaz-C1

    Plasmid
    #54744
    Purpose
    Localization: C1 Cloning Vector, Excitation: 514, Emission: 527
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Topaz
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. JRed-C1

    Plasmid
    #54788
    Purpose
    Localization: C1 Cloning Vector, Excitation: 584, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    JRed
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. JRed-N1

    Plasmid
    #54789
    Purpose
    Localization: N1 Cloning Vector, Excitation: 584, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    JRed
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. AsRed2-N1

    Plasmid
    #54703
    Purpose
    Localization: N1 Cloning Vector, Excitation: 576, Emission: 592
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    AsRed2
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pENTR-THT

    Plasmid
    #55790
    Purpose
    GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Promoter
    modified H1 RNA gene
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    55790-plasmid-map-sequence-id-89572

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  12. pTRC-LIC

    Plasmid
    #62343
    Purpose
    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    tac promoter from pKK233-2
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62343-plasmid-map-sequence-id-102266

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  14. HcRed1-CytERM-N-17

    Plasmid
    #55840
    Purpose
    Localization: N1 Cloning Vector, Excitation: 588, Emission: 618
    Depositor
    Michael Davidson
    Insert
    CytERM
    Tags
    HcRed1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55840-plasmid-map-sequence-id-221613

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  15. pICSL13008

    Plasmid
    #245656
    Purpose
    Level 0 Golden Gate part Long 35s gene cauliflower mosaic virus and 5 prime untranslated leader tobacco mosaic virus promoter, GGAG - CCAT
    Depositor
    Mark Youles
    Insert
    Long 35s gene cauliflower mosaic virus and 5 prime untranslated leader tobacco mosaic virus promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245656-plasmid-map-sequence-id-495550

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  16. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    104910-plasmid-map-sequence-id-206061

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  17. p663-UBC-miniTurbo-V5-PIP5K1A_IDG-K

    Plasmid
    #214462
    Purpose
    Gateway destination clone of PIP5K1A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-PIP5K1A (PIP5K1A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214462-plasmid-map-sequence-id-425204

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  18. p663-UBC-miniTurbo-V5-RIOK1_IDG-K

    Plasmid
    #214464
    Purpose
    Gateway destination clone of RIOK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-RIOK1 (RIOK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214464-plasmid-map-sequence-id-423214

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  19. p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K

    Plasmid
    #214459
    Purpose
    Gateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CSNK1G2 (CSNK1G2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214459-plasmid-map-sequence-id-423212

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  20. pInt_attP1_LCS_kanR

    Plasmid
    #205300
    Purpose
    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205300-plasmid-map-sequence-id-407004

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Showing: 8181 - 8200 of 62186 results