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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,036 results for: mEGFP

Showing: 841 - 860 of 1036 results

  1. pUC57-Amp_CRISPIE donor B5

    Plasmid
    #172846
    Purpose
    CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172846-plasmid-map-sequence-id-340979

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  2. pX330 _CRISPIE donor B10

    Plasmid
    #172851
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172851-plasmid-map-sequence-id-341020

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  4. pX330 _HITI donor B6

    Plasmid
    #172847
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172847-plasmid-map-sequence-id-341030

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  8. pAAV_ACTB CRISPIE

    Plasmid
    #172853
    Purpose
    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Inserts
    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172853-plasmid-map-sequence-id-341002

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  9. pcDNA3.1-CHRFAM7ADelta2bp-mGFP

    Plasmid
    #62636
    Purpose
    mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loop
    Depositor
    Henry Lester
    Article
    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6
    Insert
    CHRFAM7A (CHRFAM7A Human)
    Use
    Tags
    mEGFP inserted in M3-M4 loop
    Expression
    Mammalian
    Mutation
    there is a 2-bp deletion in exon 6 of CHRFAM7A ca…
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. EGFP-QQN

    Plasmid
    #193563
    Purpose
    Expresses human Clathrin Light Chain B mutant (residues 20-22 were substituted from EED to QQN) tagged with mEGFP in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Changed from EED to QQN at residue 20-22
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193563-plasmid-map-sequence-id-380159

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  12. CLC-EGFP206(5)

    Plasmid
    #193573
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 5 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193573-plasmid-map-sequence-id-380171

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  13. CLC-EGFP206(6)

    Plasmid
    #193574
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 6 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193574-plasmid-map-sequence-id-380173

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  14. CLC-EGFP206(7)

    Plasmid
    #193575
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 7 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193575-plasmid-map-sequence-id-383667

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  15. CLC-EGFP206(flex5)

    Plasmid
    #193576
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 5 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193576-plasmid-map-sequence-id-380174

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  16. CLC-EGFP206(flex6)

    Plasmid
    #193577
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 6 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193577-plasmid-map-sequence-id-380176

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  17. CLC-EGFP206(flex7)

    Plasmid
    #193578
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 7 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193578-plasmid-map-sequence-id-383666

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  18. pHR-PD-1 (BTLA ITIM)-mGFP

    Plasmid
    #180817
    Purpose
    Expression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFP
    Depositor
    Enfu Hui
    Article
    Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1
    Insert
    PD-1 (BTLA ITIM) (PDCD1 Human)
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mutation
    replaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…
    Promoter
    SFFV
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    180817-plasmid-map-sequence-id-356697

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  19. TGN38 EGFP

    Plasmid
    #128148
    Purpose
    Expression of TGN38 amino acids 304-357
    Depositor
    Jennifer Lippincott-Schwartz
    Insert
    TGN38 (Tgoln2 Rat)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Contains amino acids 304-357
    Promoter
    Available since
    Nov. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128148-plasmid-map-sequence-id-251067

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  20. pCAG-2dV-Camui (wt)

    Plasmid
    #220366
    Purpose
    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)
    Depositor
    Ryohei Yasuda
    Article
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    Use
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220366-plasmid-map-sequence-id-434512

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Showing: 841 - 860 of 1036 results