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pRS423-ProSgH

Plasmid

#163974

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BXG-altTAG

Plasmid

#158146

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (w/ TAG codon)

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BXG

Plasmid

#158145

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (w/ TAG codon)

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BYG

Plasmid

#158144

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (wild-type)

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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plIVMD163

Plasmid

#160935

Purpose

In vivo mRNA display construct for mCherry

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEM03

Plasmid

#135417

Purpose

Expression of Hygromycin resistance from Spizellomyces Hsp70 promoter

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr

Expression

Bacterial and Yeast

Promoter

Hsp70pr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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S3_001_026_096

Plasmid

#134402

Purpose

pYTK096-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pTDH3

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMET17p-CCG 316

Plasmid

#131166

Purpose

For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21222-FLP-3xFLAG

Plasmid

#109165

Purpose

Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

Insert

Flp

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSM002

Plasmid

#122936

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML043C (Marked)

Expression

Yeast

Mutation

YML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATAC

Promoter

[tetO2]4inPgal1

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSM001

Plasmid

#122935

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML029W (Marked)

Expression

Yeast

Mutation

YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

Promoter

[tetO2]4inPgal1

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEC005

Plasmid

#122939

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML071C (Marked)

Expression

Yeast

Mutation

YML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGC

Promoter

[tetO2]4inPgal1

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_207

Plasmid

#122938

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML070W (Marked)

Expression

Yeast

Mutation

YML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTA

Promoter

[tetO2]4inPgal1

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_243

Plasmid

#122940

Purpose

Expression of the marked mRNA under the control of insertional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML029W (Marked)

Expression

Yeast

Mutation

YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

Promoter

Pyml029w-tetO(2)

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSM004

Plasmid

#122937

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML065W (Marked)

Expression

Yeast

Mutation

YML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAAC

Promoter

[tetO2]4inPgal1

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_228

Plasmid

#122933

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YBR025C (Marked)

Expression

Yeast

Mutation

YBR025C: bp 52-63 GGTAATAACTTG to GGAAACAATTTA

Promoter

[tetO2]4inPgal1

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_229

Plasmid

#122934

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YBR026C (Marked)

Expression

Yeast

Mutation

YBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAG

Promoter

[tetO2]4inPgal1

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1859

Plasmid

#123065

Purpose

Positive regulator of Ste20

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste50 [3]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS511

Plasmid

#123063

Purpose

Negative regulator of Ste12

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Dig2 [3]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS510

Plasmid

#123062

Purpose

Negative regulator of Ste12

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Dig1 [3]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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