159,461 results

1. hyeA3A-BE4max

   Plasmid

   #157944

   Purpose

   Mammalian expression of hyeA3A-BE4max

   Depositor

   Dali Li

   Article

   Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

   Insert

   hyeA3A-BE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5 BMPS HK318/9EE-6xHis

   Plasmid

   #204836

   Purpose

   Mammalian expression of recombinant BMPS WT

   Depositor

   Monther Abu-Remaileh

   Article

   Medoh et al Science. 2023 Sep 15;381(6663):1182-1189

   Insert

   CLN5 (CLN5 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   HK318/9EE

   Promoter

   CMV

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p236

   Plasmid

   #186182

   Purpose

   Shotgun Metagenomic Spike-in

   Depositor

   Karsten Zengler

   Article

   Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

   Insert

   synDNA 236
   

   Use

   Synthetic Biology; Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-ChRger3-TS-EYFP

   Plasmid

   #127240

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger3-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139089

   Purpose

   Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   xCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-C1-pmDH

   Plasmid

   #67897

   Purpose

   Expresses a fusion of mCherry with the membrane located DH domain (1-347) of p63RhoGEF in mammalian cells

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

   Insert

   mCherry-pmDH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKT-IDH1(R132H)-IRES-Katushka

   Plasmid

   #124257

   Purpose

   Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

   Insert

   Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Arginine 132 is mutated to Histidine. This mutati…

   Promoter

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. UbC-Halo-SFPQ

   Plasmid

   #166946

   Purpose

   Lentiviral plasmid expressing Halo-tagged SFPQ protein from the UbC promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   Halo

   Expression

   Mutation

   Promoter

   UbC

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW)

   Plasmid

   #189791

   Purpose

   Express wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

   Depositor

   Robert Singer

   Article

   Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

   Insert

   beta-globin (HBB Mouse, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ponasterone A inducible

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET11a-Z-NspGFP

    Plasmid

    #40729

    Depositor

    Brian McNaughton

    Article

    Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

    Insert

    N super positive GFP - Leucine Zipper
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV4a-Flag-c-Myc

    Plasmid

    #102625

    Purpose

    Expresses c-Myc in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA Flag ERbeta

    Plasmid

    #35562

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFLAG-CMV2-Hsp27-S15D/S78D/S82D

    Plasmid

    #85188

    Purpose

    Hsp27 expression plasmid

    Depositor

    Ugo Moens

    Insert

    Hsp27 (HSPB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S15D/S78D/S82D, phosphomimicking mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

    Plasmid

    #100157

    Purpose

    Expresses MUS81

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    MUS81 (MUS81 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3-TGFb1

    Plasmid

    #101762

    Purpose

    TGFb1 promoter reporter

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    transforming growth factor beta 1 promoter (TGFB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIVTR(T7A)-NGN2 (SA Substitutions)

    Plasmid

    #172308

    Purpose

    In vitro transcription of NGN2 (Ser phosphosites substituted by Ala)

    Depositor

    Patrik Verstreken

    Insert

    NGN2
    

    Use

    Other

    Tags

    Expression

    Mutation

    S24A, S193A, S207A, S209A, S219A, S232A, S239A an…

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQCXIG (w497-1)

    Plasmid

    #26286

    Depositor

    Eric Campeau

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mApple-Vinculin-N-21

    Plasmid

    #54962

    Purpose

    Localization: Focal Adhesions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only