169,348 results

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pEG302 22aa SunTag VP64 nog

Plasmid

#120251

Purpose

CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBW121-SS-315

Plasmid

#68786

Purpose

8x Target 1 0gap with SV40 Ori

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Insert

8x 0gap Target 1 with SV40 ori

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C

Plasmid

#184410

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CaV1.2/pcDNA3.1~ Human

Plasmid

#140576

Purpose

Expression in mammallian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

Insert

human CaV1.2 (CACNA1C Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab5A Q79L

Plasmid

#28046

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab5A (RAB5A Human)

Tags

EGFP on N-term

Expression

Mammalian

Mutation

Q79L

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

Plasmid

#190790

Purpose

[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Inserts

Cas9
mazF-LVA

Use

CRISPR and Synthetic Biology

Tags

LVA

Expression

Bacterial

Mutation

silent mutations of BpiI-sites in original Cas9 g…

Promoter

PnrsB and Ptrc-[E*](104)

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.26-SS-192

Plasmid

#68759

Purpose

ZF9 Op x6 -- GFP

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Insert

ZF9 Op 6x

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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paavCAG-post-mGRASP-2A-dTomato

Plasmid

#34912

Purpose

post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Has Service

AAV5

Insert

postsynaptic mGRASP

Use

AAV

Promoter

CAG

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-GCaMP6F

Plasmid

#137122

Purpose

Intersectional viral expression of GCaMP6F in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV5 and AAV8

Insert

Con/Fon-GCaMP6F

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSCAR_Cas9-hygro_GFP

Plasmid

#162075

Purpose

3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Inserts

Cas9-2a-hygromycin B phosphotransferase
EGFP

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EF1a and sv40

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE-tdTomato

Plasmid

#50798

Purpose

Expression of tdTomato under the control of the TRE-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

TREtight driven tdTomato fluorescent protein

Use

Mutli-species

Promoter

TREtight

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hATG5 WT

Plasmid

#111803

Purpose

Mammalian Expression of hATG5

Depositor

Jun Hee Lee

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

hATG5 (ATG5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-HaloTag-CNA35

Plasmid

#215457

Purpose

Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

CNA35

Tags

HaloTag

Expression

Bacterial

Mutation

Includes stop codon

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1-GW ymYPET

Plasmid

#118455

Purpose

Overexpression of ymYPET

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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HO-hisG-URA3-hisG-poly-HO

Plasmid

#51663

Purpose

yeast plasmid for integration of target sequence at HO locus, URA3 selection

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pPGKpuro

Plasmid

#11349

Depositor

Rudolf Jaenisch

Article

Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

Insert

pgk-1 5' and 3' regions (Pgk1 Mouse)

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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LifeAct-GFP-Fis1

Plasmid

#182576

Purpose

Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

LifeAct-GFP-Fis1

Tags

Fis1 and LifeAct

Expression

Mammalian

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Puro DEST (670-1)

Plasmid

#17293

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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mVenus-H2B-6

Plasmid

#56615

Purpose

Localization: Nucleus/Histones, Excitation: 515, Emission: 528

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

mVenus

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEos3.2-N1

Plasmid

#54525

Purpose

Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson, Tao Xu

Article

Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

Type

Empty backbone

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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