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AiP12360 - pAAV-AiE0519h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2360)

Plasmid

#223986

Purpose

AiE0519h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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ER-RA

Plasmid

#209872

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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tdTomato-anti-AlfaTag nanobody

Plasmid

#171819

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

tdTomato-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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FUCas9Cherry

Plasmid

#70182

Purpose

Expresses Cas9 with fluorescent Cherry reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

humanised S.pyogenes cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

hUbC

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pOlyA(E69A)

Plasmid

#132392

Purpose

Mutant version of OlyA that senses sphingomyelin without or with cholesterol

Depositor

Arun Radhakrishnan

Article

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A point mutant (E69A)

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, E69A, C94S, S151C

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLBH531_MBP-Cas14a1 expression

Plasmid

#112500

Purpose

Expresses MBP fused to Cas14a1

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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Red cGull

Plasmid

#191853

Purpose

Expresses biosensor for cGMP in mammalian cells

Depositor

Takashi Tsuboi

Article

Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

Insert

Red cGull

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3412

Plasmid

#144888

Purpose

Lentiviral vector for overexpressing the EGR1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EGR1 (EGR1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCXLE-gw

Plasmid

#37626

Purpose

Non-integrating (episomal) expression vector with Gateway cassette

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Expression

Bacterial

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-TRE>{MCS}:T2A:EGFP

Plasmid

#236250

Purpose

Hygromycin selected lentiviral vector with MCS for tetracycline-inducible gene, for use with dual-inducible CymR-CuO system

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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T-2A-EGFP-PGK-Puro

Plasmid

#83344

Purpose

Donor vector to knocin EGFP into human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

Human Brachyury knockin homology arms (TBXT Human)

Tags

2A-EGFP

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSEM246 - mosTI - unc-119 - MCS

Plasmid

#182343

Purpose

Empty cloning vector for mosTI(unc-119). MCS or Golden Gate (BsaI: tgcc - MCS - gagc)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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mRuby-N1

Plasmid

#54581

Purpose

Localization: N1 Cloning Vector, Excitation: 558, Emission: 605

Depositor

Michael Davidson

Type

Empty backbone

Tags

mRuby

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET-21a(+)-IPP1-His

Plasmid

#118978

Purpose

Bacterial expression plasmid for Inorganic pyrophosphatase

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

inorganic diphosphatase IPP1 (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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dCas12j2 KRAB

Plasmid

#188508

Purpose

Expresses FLAG tagged dCas12j2 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERP2

Plasmid

#80477

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-M337V

Plasmid

#133759

Purpose

Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed methionine 337 to valine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pWH1266-Apra-sgRNA

Plasmid

#218372

Purpose

sgRNA expression plasmid for CRISPR-intereference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

sgRNA expression cassette

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-Lime

Plasmid

#191255

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein Lime

Depositor

Robert Campbell

Article

Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

Insert

Lime

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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