166,033 results

1. pTF101.3

   Plasmid

   #134772

   Purpose

   A binary plasmid containing double CaMV 35S promoter to drive bialophos resistance gene (bar) for plant transformation.

   Depositor

   Kan Wang

   Article

   Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE TRE dCas9-VP64

   Plasmid

   #50916

   Purpose

   Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A nuclease-deficient

   Promoter

   TRE

   Available Since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEmpty_Control

   Plasmid

   #162607

   Purpose

   Empty control for Cas9 experiments and to clone new guide RNAs for paper

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Expression

   Yeast

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a_KLF17_P2A_Hygro_Barcode

   Plasmid

   #120503

   Purpose

   Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF17 (KLF17 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNL [NlucP/STAT6-RE/Hygro] Vector

   Plasmid

   #236925

   Purpose

   Express STAT6 response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   STAT6 (STAT6 Human)
   

   Use

   Luciferase

   Tags

   NanoLuc (R)

   Mutation

   None

   Promoter

   minP

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. T10-T7-hMOV10

   Plasmid

   #185052

   Purpose

   expression of WT human MOV10

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   cDNA WT hMOV10 (MOV10 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CROP_C9_Puro_DNMT1

   Plasmid

   #183282

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets DNMT1 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   DNMT1
   

   Use

   Lentiviral

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-EGFP-eDHFR(69K6)-MCS

   Plasmid

   #172100

   Purpose

   Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)-20aa
   

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. gh123

   Plasmid

   #106790

   Purpose

   expression of gRNA targeting ST8SIA3

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   ST8SIA3 (ST8SIA3 Human)
   

   Expression

   Mammalian

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Casp8

    Plasmid

    #11817

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 8 (CASP8 Human)
    

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHSN6A01

    Plasmid

    #50586

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    2×35Sp and AtU6-26p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-CASANOVA

    Plasmid

    #113035

    Purpose

    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTEI071

    Plasmid

    #105376

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    promotor CAB3 (At1g29910; 1000bp)
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT_TO_eGFP_TIA1

    Plasmid

    #106094

    Purpose

    gene expression of EGFP-TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIA1 (TIA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX_EFS-Cre_ppt-del

    Plasmid

    #162073

    Purpose

    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EFS (part of insert)

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid

    #197503

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP VPS41

    Plasmid

    #245032

    Purpose

    Protein localization of human wild-type VPS41 in cells

    Depositor

    Thomas Martin

    Insert

    VPS41 (VPS41 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. PS Intein

    Plasmid

    #242019

    Purpose

    Blue-light-induced reconstitution of split mCherry.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    PS Intein
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. PS Intein/C1A Bid

    Plasmid

    #242025

    Purpose

    PS Intein Bid construct containing the C1A mutation in gp41-1, which abolishes its autocatalytic splicing activity.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    PS Intein/C1A Bid
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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