163,606 results

1. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105 and tet

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP29-1D(sfGFP)

   Plasmid

   #126963

   Purpose

   Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJUMP29-1C(sfGFP)

   Plasmid

   #126962

   Purpose

   Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJUMP29-1B(sfGFP)

   Plasmid

   #126961

   Purpose

   Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-DAXX-D/E-His

   Plasmid

   #179619

   Purpose

   Express DAXX D/E region in bacteria

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   GST, His, and TEV

   Expression

   Bacterial

   Mutation

   amino acids 414-505 of DAXX

   Promoter

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X

   Plasmid

   #192151

   Purpose

   T cell CRISPR AAV-SB vector

   Depositor

   Sidi Chen

   Article

   Ye et al Nat Biotechnol. 2019 Nov;37(11):1302-131

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTK_Sox10_enh_99a_Citrine

   Plasmid

   #130589

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   Sox10_enh_99a
   

   Use

   Enhancer reporter

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. EC2_1_dCas9_sgRNA

   Plasmid

   #163710

   Purpose

   Yeast low copy plasmid with dCas9 and sgRNA expression cassette

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-miSFIT-2x

   Plasmid

   #124691

   Purpose

   Tuning expression of EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid

    #61364

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJUMP29-1E(sfGFP)

    Plasmid

    #126982

    Purpose

    Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCheY Tc delta C

    Plasmid

    #13520

    Depositor

    James McKerrow

    Article

    Eakin et al J Biol Chem. 1993 Mar 25. 268(9):6115-8.

    Insert

    cruzain
    

    Use

    Tags

    CheY

    Expression

    Bacterial

    Mutation

    Truncated variant: stop codon after Gly212

    Promoter

    Available Since

    March 11, 2009

    Availability

    Academic Institutions and Nonprofits only

13. XP7 Sonic hh

    Plasmid

    #16983

    Depositor

    Randall Moon

    Insert

    sonic hedgehog
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pJUMP49-2D(sfGFP)

    Plasmid

    #126967

    Purpose

    Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJUMP49-2C(sfGFP)

    Plasmid

    #126966

    Purpose

    Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJUMP49-2B(sfGFP)

    Plasmid

    #126965

    Purpose

    Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-Synapto-pHluorin

    Plasmid

    #168510

    Purpose

    Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    Synaptophysin-pHluorin (Syp Mouse)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCB6-EnvA

    Plasmid

    #74420

    Purpose

    Mammalian expression of the subgroup A avian leukosis-sarcoma envelope glycoprotein (EnvA)

    Depositor

    Judith White

    Article

    Gilbert et al J Virol. 1994 Sep;68(9):5623-8.

    Insert

    Env from ASLV-A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pHAGE-CDK12

    Plasmid

    #116723

    Purpose

    Lentiviral expression of CDK12

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CDK12 (CDK12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS xChordin

    Plasmid

    #24975

    Depositor

    Edward De Robertis

    Article

    Sasai et al Cell. 1994 Dec 2. 79(5):779-90.

    Insert

    Chordin (chrd.1.S Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only