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Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies.
Tang JC, Drokhlyansky E, Etemad B, Rudolph S, Guo B, Wang S, Ellis EG, Li JZ, Cepko CL
Elife. 2016 May 20;5. pii: e15312. doi: 10.7554/eLife.15312.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
75469pAAV-EF1a-Flp-DOG-NWFlp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells
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75470pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherryFlp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells
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80084pCAG-Flp-DOGFlp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells
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80086pCAG-dGBP1-TagBFPGFP-dependent dGBP1-TagBFP, for expression in mammalian cells
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80087pBMN-dGBP1-TagBFPMMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker
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