pBMN-dGBP1-TagBFP
(Plasmid #80087)

• Purpose: MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker
• Depositing Lab: Connie Cepko
• Publication: Tang et al Elife. 2016 May 20;5. pii: e15312. doi: 10.7554/eLife.15312.

Backbone

• Vector backbone: pBMN-DHFR(DD)-YFP
• Backbone manufacturer: Thomas Wandless
• Backbone size w/o insert (bp): 6944
• Total vector size (bp): 8003
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: IRES-tHcRed

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C. (From pBMN DHFR(DD)YFP page)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: dGBP1-TagBFP
• Species: Synthetic
• Insert Size (bp): 1059

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pBMN 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please cite: Tang JC, Drokhlyansky E, Etemad B, Rudolph S, Guo B, Wang S, Ellis EG, Li JZ, Cepko CL (2016) Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. Elife 5. doi:10.7554/eLife.15312