163,606 results

1. pcDNA3-FKBP-EGFP-HOTag3

   Plasmid

   #106924

   Purpose

   Expresses FKBP-EGFP-HOTag3 in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   FKBP-EGFP-HOTag3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. sUPRa

   Plasmid

   #223242

   Purpose

   sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

   Depositor

   Colin Akerman

   Article

   Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

   Insert

   sUPRa
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Custom

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETMCSI-T4P_Y320A

   Plasmid

   #122522

   Purpose

   Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

   Depositor

   Gottfried Otting

   Article

   Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

   Insert

   gp43 - DNA polymerase (43 Escherichia virus T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   tyrosine 320 changed to alanine

   Promoter

   T7 promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Tet1

   Plasmid

   #60938

   Purpose

   Expresses Tet1 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KLC2

   Plasmid

   #40735

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   KLC2 (KLC2 Human)
   

   Use

   Tags

   6xHis TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

6. ERM-APEX2

   Plasmid

   #79055

   Purpose

   Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 anchored to the cytosolic face of the ER membrane
   

   Use

   Lentiviral

   Tags

   Residues 1-27 of P450 oxidase 2C1 and V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV immearly promotor

   Available Since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEYFP-NR1a

   Plasmid

   #17928

   Depositor

   Stefano Vicini

   Article

   Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

   Insert

   N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHM092

   Plasmid

   #225526

   Purpose

   Encodes rtTA under AR8 promoter, and constitutive BFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Inserts

   rtTA
   BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHM093

   Plasmid

   #225525

   Purpose

   Encodes rtTA under IBRE4 promoter, and constitutive BFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Inserts

   rtTA
   BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHM039

    Plasmid

    #225523

    Purpose

    Encodes Wnt-responsive rtTA and constitutive BFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    rtTA
    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pdest METTL1-WT Neo

    Plasmid

    #223058

    Purpose

    METTL1 gene expression in mammalian cells

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    METTL1 (METTL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCI-M2

    Plasmid

    #44170

    Depositor

    John Olsen

    Article

    Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

    Insert

    Influenza A virus M2 matrix gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LeGO-EBFP2

    Plasmid

    #85213

    Purpose

    Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    EBFP2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.3-SARS2-B.1.617.2

    Plasmid

    #172320

    Purpose

    expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.2 strain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

    Promoter

    CMV-F

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Flag-NPM1wt

    Plasmid

    #131810

    Purpose

    Expresses Flag-NPM1wildtype (human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EF.STAT3DN.Ubc.GFP

    Plasmid

    #24984

    Purpose

    Depositor

    Linzhao Cheng

    Article

    Hillion et al Cancer Research 68, 10121, December 2008

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y705F--dominant negative

    Promoter

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSRE SSG

    Plasmid

    #127692

    Purpose

    Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Structural Genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. AYBEv3

    Plasmid

    #193967

    Purpose

    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G163R, N169S, S198A, K202A, G203A, S206A, K210A

    Promoter

    hU6, EF1a, CBh

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

    Plasmid

    #216668

    Purpose

    Expresses membrane-bound mCherry in mammalian cells

    Depositor

    Leonardo Morsut

    Insert

    mCherry
    

    Use

    Tags

    PDGFR Transmembrane Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF1a_ESRRG_P2A_Hygro_Barcode

    Plasmid

    #120435

    Purpose

    Barcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ESRRG (ESRRG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge