161,709 results

1. pCOLA-Gent-EM7-Erv1p-PDI

   Plasmid

   #202486

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   EM7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-6xHis-MBP-TEV site-TadA8r

   Plasmid

   #208318

   Purpose

   Overxpression MBP-TadA8r

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-CjCas9

   Plasmid

   #89754

   Purpose

   Expression of CjCas9 with His tag in E.coli

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   6XHis, HA, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCMV-SS-antiHAscFv-TM

   Plasmid

   #198302

   Purpose

   Displays anti-HA scFv from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiHAscFv-TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. evoBE4-SpRY

   Plasmid

   #226854

   Purpose

   Expresses evoBE4max-SpRY base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   evoBE4max-SpRY
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. shh10

   Plasmid

   #64867

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

   Depositor

   David Schaffer, John Flannery

   Article

   Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

   Insert

   shh10 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   I319V, N451D, D532N

   Promoter

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-DIO-ChR2(H134R)-eYFP

   Plasmid

   #127090

   Purpose

   Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation

   Depositor

   Viviana Gradinaru

   Article

   Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

   Has Service

   AAV PHP.eB

   Insert

   Channelrhodopsin-2
   

   Use

   AAV

   Tags

   eYFP (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Humanized ChR2 gene with histidine 134 changed to…

   Promoter

   CAG

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-PBRM1

   Plasmid

   #65387

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   PBRM1 (PBRM1 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-rab7 DN

   Plasmid

   #12660

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB7 (Homo sapiens) (RAB7A Human)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   T22N

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDARMO_HUWE1_3xFLAG

    Plasmid

    #187155

    Purpose

    mammalian cell expression of HUWE1-3xFLAG

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. IMPT-10314:GABBR2

    Plasmid

    #157851

    Purpose

    HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

    Depositor

    Raymond Stevens, Vadim Cherezov

    Article

    Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

    Insert

    GABBR2 (GABBR2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-sumo-h-cGAS

    Plasmid

    #127161

    Purpose

    expression of human cGAS protein

    Depositor

    Thomas Tuschl

    Article

    Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

    Insert

    MB21D1 (CGAS Human)
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdRBFC-NS1-YN

    Plasmid

    #74723

    Purpose

    Express C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling

    Depositor

    Aiming Wang

    Article

    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

    Insert

    Influenza A virus NS1 protein, partial
    

    Use

    Double-stranded rna labeling

    Tags

    HA and YFP N-terminal half domain

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSGV Mouse Acceptor

    Plasmid

    #128176

    Purpose

    A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. GPER-DuET

    Plasmid

    #213256

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPER (GPER1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. opto-RhoA-mCherry_pcDNA3.1

    Plasmid

    #164472

    Purpose

    Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

    Insert

    opto-RhoA
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1+_Lambda-N-HA-Peptide

    Plasmid

    #92005

    Purpose

    Expresses Lambda-N-HA peptide

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    Lambda-N-HA peptide
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-myrAkt-P2A-BCL2

    Plasmid

    #233182

    Purpose

    Retroviral expression of myristoylated Akt1 (myr-Akt) and Bcl2

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    myr-Akt (Akt1, BCL2 Mouse, Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    myr

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-opto-mCasp1

    Plasmid

    #208781

    Purpose

    Inducibly expresses mouse caspase1 fused to light activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mCaspase1 Cry2 (Casp1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry, Cry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. spNW80

    Plasmid

    #173487

    Purpose

    Expresses spNW80 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW80
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge