159,481 results

1. COC053

   Plasmid

   #209131

   Purpose

   Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

   Depositor

   Christopher A. O'Callaghan

   Article

   O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

   Insert

   Enzyme BirA (birA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEGFP

   Plasmid

   #18696

   Depositor

   Karel Svoboda

   Article

   Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

   Insert

   monomeric EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K mutation

   Promoter

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIS1-Eef25UTR-renilla

   Plasmid

   #38235

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2 5'UTR / Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Eef2 5' UTR fused to renilla ORF

   Promoter

   Endogenous Eef2

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 FLII12Pglu-700uDelta6

   Plasmid

   #17866

   Depositor

   Wolf Frommer

   Article

   Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

   Insert

   FLII12Pglu-700uDelta6
   

   Use

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   700uDelta6, ECFP insert in mglB

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2Flag-pDEST-C

   Plasmid

   #118372

   Purpose

   Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   2Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKG123

   Plasmid

   #63074

   Purpose

   Retroviral expression of mCherry-TEV-S-tag CENP-C for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   CENP-C (CENPC Human)
   

   Use

   Retroviral

   Tags

   TEV-S and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTARA

   Plasmid

   #31491

   Depositor

   Kathleen Matthews

   Article

   Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

   Insert

   RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N823D

   Promoter

   Available since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. CL20_mEGFP_KMT2A_MLLT1

   Plasmid

   #205842

   Purpose

   Express mEGFP-tagged fusion protein, KMT2A_MLLT1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   KMT2A_MLLT1 (KMT2A, MLLT1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_VNp15-mNeongreen

   Plasmid

   #182389

   Purpose

   Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp15-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5 HA-TGFBRII

    Plasmid

    #19158

    Depositor

    Joan Massague

    Insert

    Transforming growth factor, beta receptor II (TGFBR2 Human)
    

    Use

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    PCR based mutagenesis

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-FNLS-P2A-Puro

    Plasmid

    #110841

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-TK-5UTR-BsmBI-Luciferase

    Plasmid

    #114670

    Purpose

    For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

    Depositor

    Lynne Postovit

    Article

    Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

    Insert

    LacZ
    

    Use

    Luciferase

    Tags

    for cloning 5' UTR-Luciferase fusions

    Expression

    Mammalian

    Mutation

    Promoter

    lac promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. inert_EJC

    Plasmid

    #216224

    Purpose

    GFP P2A RFP reporter with inert EJC immediately following stop codon

    Depositor

    Rebecca Voorhees

    Article

    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

    Insert

    GFP P2A RFP_inert_EJC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3_mScarlet-LAMP1

    Plasmid

    #185138

    Purpose

    Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

    Depositor

    Andrew York

    Article

    Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

    Insert

    Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-21 of LAMP1

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PE5max-SF3B1-K700E

    Plasmid

    #200317

    Purpose

    Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Inserts

    K700E-ng1+pg2
    PEmax-2A-MLH1dn
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Str-KDEL_TNF-SBP-EGFP

    Plasmid

    #65278

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-GFP11-His3MX

    Plasmid

    #91951

    Purpose

    Universal C-terminal GFP11 tagging

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)/Luc2=tdT

    Plasmid

    #32904

    Purpose

    Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

    Depositor

    Christopher Contag

    Article

    Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

    Inserts

    Firefly Luciferase
    tandem Tomato Red Fluorescent Protein
    

    Use

    Tags

    tdTomato red fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE and n/a

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Ii-OVA.I+II

    Plasmid

    #64596

    Purpose

    Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation

    Depositor

    Martin Zenke, Sandra Diebold

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Invariant chain

    Expression

    Mammalian

    Mutation

    Only amino acids 242-353 (class I and class II ep…

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-TLR5-CFP

    Plasmid

    #13019

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 5 (TLR5 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge