163,607 results

1. pL1P3 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198047

   Purpose

   MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available Since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

   Plasmid

   #131004

   Purpose

   bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   Kv2.1-HA

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p101_CRISPRai_PiggyBac

   Plasmid

   #213777

   Purpose

   Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   VPR-dSaCas9
   dSpCas9-KRAB-BFP
   zeocin
   Tet-On transactivator
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a (EF-1-alpha intron a) and Tet_On_TRE

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCAR_Cas9-blast_GFP

   Plasmid

   #162074

   Purpose

   3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Inserts

   Cas9-2a-blasticidin S deaminase
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in M. musculus

   Promoter

   EF1a and sv40

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. iCAP-BI-28-KanR

   Plasmid

   #203532

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI28 Cap
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK_TFAP2b_enh_226_Citrine

   Plasmid

   #130618

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   TFAP2b_enh_226
   

   Use

   Enhancer reporter

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. sg2.02 16x(MS2) MUC4.1

   Plasmid

   #101154

   Purpose

   gRNA with 16 MCP binding site

   Depositor

   Mazhar Adli

   Article

   Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

   Insert

   MUC4 (MUC4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-TAF4B

   Plasmid

   #192927

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of TAF4B

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   TAF4B (TAF4B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCHERRY3

   Plasmid

   #24659

   Depositor

   Tanya Parish

   Article

   Carroll et al PLoS One. 2010 . 5(3):e9823.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2FA-H2B-mScarlet3

    Plasmid

    #224436

    Purpose

    Construct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    H2B-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV & SP6

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-eGFP-C1-VPS35

    Plasmid

    #163622

    Purpose

    human VPS35 cDNA C-terminally tagged with eGFP

    Depositor

    Josef Kittler

    Article

    Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

    Insert

    human VPS35 (VPS35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Nluc

    Plasmid

    #180501

    Purpose

    Mammalian expression of Nluc

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Nluc
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJUMP49-2A(sfGFP)

    Plasmid

    #126964

    Purpose

    Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p52sheLL

    Plasmid

    #46950

    Purpose

    HPV52 L1 and L2 Bicistronic, too large to self-package

    Depositor

    John Schiller

    Article

    Kondo et al J Med Virol. 2008 May;80(5):841-6. doi:

    Insert

    HPV52L1+L2 (L1, L2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBV-Luc wt MBS1-4

    Plasmid

    #16564

    Depositor

    Bert Vogelstein

    Article

    Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

    Insert

    c-MYC binding sites (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDIRECT_10A

    Plasmid

    #91207

    Purpose

    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJUMP29-1A(sfGFP)

    Plasmid

    #126960

    Purpose

    Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpliceExpress

    Plasmid

    #32485

    Depositor

    Stefan Stamm

    Article

    Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSIR-bsr

    Plasmid

    #176507

    Purpose

    Self-inactivating retrovirus vector containing a Blasticidin S-resistance gene

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2021 Dec 6;6(1):bpa

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDESTtol2pACrymCherry

    Plasmid

    #64023

    Purpose

    Gateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lens

    Depositor

    Peter Currie, Joachim Berger

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    mCherry
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    alpha-Acrystallin promoter

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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