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Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity.
Moreb EA, Hoover B, Yaseen A, Valyasevi N, Roecker Z, Menacho-Melgar R, Lynch MD
ACS Synth Biol. 2017 Oct 2. doi: 10.1021/acssynbio.7b00174.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
89951pKDsgRNA-malGContains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.
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89952pKDsgRNA-arsBContains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.
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89953pKDsgRNA-rpsLContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.
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89954pKDsgRNA-hisGContains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.
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89955pKDsgRNA-trmIContains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.
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89956pKDsgRNA-torZContains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.
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89957pKDsgRNA-rssAContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.
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89958pKDsgRNA-yhhTContains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.
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89959pKDsgRNA-lpoBContains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.
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89960pKDsgRNA-NTContains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.
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89961pEM-Cas9HF1Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
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89962pEM-Cas9HF1-recA56Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.
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89963pKDsgRNA-rbsKContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.
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89964pKDsgRNA-xylAContains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.
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89965pKDsgRNA-srlAContains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.
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102283pSMART-SOS-GFPuvFluorescent sensor of E. coli SOS response
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102284pKDsgRNA-pfkBContains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.
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102285pEM-Cas9noSsrAModified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.
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102286pKDsgRNA-fsaAContains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.
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102288pKDsgRNA-ldhAContains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.
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102290pKDsgRNA-adhEContains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.
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102291pKDsgRNA-ompTContains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.
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102294pEM-Cas9HF1-indRecA56Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.
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