Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity.
Moreb EA, Hoover B, Yaseen A, Valyasevi N, Roecker Z, Menacho-Melgar R, Lynch MD
ACS Synth Biol. 2017 Oct 2. doi: 10.1021/acssynbio.7b00174. PubMed Article
Moreb EA, Hoover B, Yaseen A, Valyasevi N, Roecker Z, Menacho-Melgar R, Lynch MD
ACS Synth Biol. 2017 Oct 2. doi: 10.1021/acssynbio.7b00174. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89951 | pKDsgRNA-malG | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG. |
| 89952 | pKDsgRNA-arsB | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB. |
| 89953 | pKDsgRNA-rpsL | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL. |
| 89954 | pKDsgRNA-hisG | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG. |
| 89955 | pKDsgRNA-trmI | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI. |
| 89956 | pKDsgRNA-torZ | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ. |
| 89957 | pKDsgRNA-rssA | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA. |
| 89958 | pKDsgRNA-yhhT | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT. |
| 89959 | pKDsgRNA-lpoB | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB. |
| 89960 | pKDsgRNA-NT | Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning. |
| 89961 | pEM-Cas9HF1 | Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016. |
| 89962 | pEM-Cas9HF1-recA56 | Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair. |
| 89963 | pKDsgRNA-rbsK | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK. |
| 89964 | pKDsgRNA-xylA | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA. |
| 89965 | pKDsgRNA-srlA | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA. |
| 102283 | pSMART-SOS-GFPuv | Fluorescent sensor of E. coli SOS response |
| 102284 | pKDsgRNA-pfkB | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB. |
| 102285 | pEM-Cas9noSsrA | Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9. |
| 102286 | pKDsgRNA-fsaA | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA. |
| 102288 | pKDsgRNA-ldhA | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA. |
| 102290 | pKDsgRNA-adhE | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE. |
| 102291 | pKDsgRNA-ompT | Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT. |
| 102294 | pEM-Cas9HF1-indRecA56 | Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair. |