159,481 results

1. pcDNA3.1-Beta3

   Plasmid

   #140988

   Purpose

   Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GBeta3 (GNB3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-GfaABC1D-GRAB_ATP1.0

   Plasmid

   #167579

   Purpose

   Express the ATP sensor GRAB_ATP1.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP sensor GRAB_ATP1.0 (P2RY1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. UAS-FRT-GFP-FRT-u(M)Cas9

   Plasmid

   #127388

   Purpose

   Cas9 inducible by removal of a GFP flip-out cassette

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   FRT-GFP-FRT-uORF(M)Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM461

   Plasmid

   #108320

   Purpose

   For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Inserts

   Che9C RecT
   Bxb1 Integrase
   Tet Repressor
   SacRB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET30B-PTEN

   Plasmid

   #20741

   Depositor

   Alonzo Ross

   Article

   Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2009

   Availability

   Academic Institutions and Nonprofits only

6. GFP-UBR5

   Plasmid

   #52050

   Purpose

   CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

   Depositor

   Darren Saunders

   Article

   Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

   Insert

   UBR5 (UBR5 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQF2WB

   Plasmid

   #61312

   Purpose

   Enhancer trap version of QF2, analogous to pGAWB

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2911

   Plasmid

   #143666

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFIC (NFIC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6c:sgRNA#4

   Plasmid

   #64248

   Purpose

   expresses sgRNA under U6c promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. tetO-MITF

    Plasmid

    #170695

    Purpose

    doxycycline-inducible overexpression of MITF

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    MITF (MITF Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

    Plasmid

    #170048

    Purpose

    Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet
    COmScarlet
    mRuby3
    mWasabi dN
    mWasabi CO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-TPH2-Cre

    Plasmid

    #189616

    Purpose

    Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Cre Recombinase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tph2

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AcGFP-MSH3

    Plasmid

    #215122

    Purpose

    Expresses AcGFP-MSH3 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    MSH3 (MSH3 Human)
    

    Use

    Retroviral

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOTTC374 - pAAV EF1a DIO iRFP

    Plasmid

    #47626

    Purpose

    An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Infrared fluorescent protein
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX_305

    Plasmid

    #41390

    Purpose

    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

    Depositor

    David Root

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mRFP-FKBP12

    Plasmid

    #67514

    Purpose

    FKBP12 only

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12 (FKBP1A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tdTomato-Keratin-17

    Plasmid

    #58106

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

19. LiPOP2

    Plasmid

    #168266

    Purpose

    A single-component optogenetic tool for photoswitchable pyroptosis

    Depositor

    Yubin Zhou

    Article

    He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

    Insert

    mGSDMD(1-276) (Gsdmd Mouse)
    

    Use

    Tags

    cpLOV2, mCherry

    Expression

    Mammalian

    Mutation

    R138A, K146A, R152A, R154A

    Promoter

    CMV

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. psPAX2-D64V-NC-COM

    Plasmid

    #131226

    Purpose

    Expressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)

    Depositor

    Baisong Lu

    Article

    Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

    Insert

    COM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge