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pAAV-DIO-IGK-dAPEX2-KDEL

Plasmid

#117183

Purpose

Plasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

IGK-dAPEX2-KDEL

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-TdTomato

Plasmid

#110338

Purpose

TdTomato reporter vector of iTango and Cal-Light system for AAV production

Depositor

Hyungbae Kwon

Insert

tetO-TdTomato

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKD001 pME minprom_EGFP

Plasmid

#195955

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann

Article

Prummel et al Nat Commun. 2019 Aug 26;10(1):3857. doi:

Insert

mouse beta-globin minimal promoter EGFP

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

Plasmid

#141378

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-CAG-flex-MCS--4x6T

Plasmid

#196420

Purpose

Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV and Cre/Lox; Astrocyte-selective

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBi2FC-C-GW-P2A:mTagBFP2

Plasmid

#216143

Purpose

Bicistronic BiFC vector (Gateway compatible)

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBi2FC-N-GW-P2A:mCherry

Plasmid

#216144

Purpose

Bicistronic BiFC vector (Gateway compatible)

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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mOrange2-pBAD

Plasmid

#54531

Purpose

Localization: Protein Expression Vector , Excitation: 549, Emission: 565

Depositor

Nathan Shaner, Roger Tsien, Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Use

Tags

6xHis and mOrange2

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pTK92_Lifeact-GFP

Plasmid

#46356

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

Lifeact

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

pBABE

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-ChrimsonR::FusionRed::Kv2.1

Plasmid

#102770

Purpose

"Activate neurons with 2p stimulation"

Depositor

Karel Svoboda

Article

Daie et al Nat Neurosci. 2021 Feb;24(2):259-265. do

Insert

ChrimsonR::FusionRed::Kv2.1

Use

AAV

Tags

FusionRed and Kv2.1 traficking domain

Expression

Mutation

Promoter

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-EWS-FLI1

Plasmid

#102813

Purpose

Overexpress EWS-FLI1. 3rd generation lentiviral vector.

Depositor

Jialiang Wang

Article

Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

Insert

EWS-FLI1 (EWSR1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB402-omega

Plasmid

#74797

Purpose

Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

2xCaMV35S enhancer and omega

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE16

Plasmid

#90357

Purpose

CREB - Cyclic AMP gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CREB

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-PTEN

Plasmid

#13039

Depositor

Alonzo Ross

Article

Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

Insert

PTEN (PTEN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC17A9_STOP

Plasmid

#161139

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC17A9 (SLC17A9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSH624-ssr

Plasmid

#199241

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

single-stranded DNA recombineering

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacIq-Ptrc

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Homer1L scFv [L113/13]

Plasmid

#190498

Purpose

Mammalian Expression of Homer1L scFV. Derived from hybridoma L113/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Homer1L (Mus musculus) recombinant scFV (Homer1 Mouse)

Use

Tags

Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-hUba2-His

Plasmid

#53117

Purpose

expression of full length human Uba2 with Cterminal His-tag in E.coli

Depositor

Frauke Melchior

Article

Werner et al Methods Mol Biol. 2009;497:187-99. doi:

Insert

UBA2 (UBA2 Human)

Use

Tags

His and T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-acceptor-2xBbsI

Plasmid

#205633

Purpose

Cloning and U6-drive expression of ISDra2 TnpB reRNAs

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(WT).ires.GFP-pcDNA3

Plasmid

#204360

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/T7 RNApol

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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