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Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing.
Viita T, Kyheroinen S, Prajapati B, Virtanen J, Frilander MJ, Varjosalo M, Vartiainen MK
J Cell Sci. 2019 Apr 17;132(8). pii: jcs.226852. doi: 10.1242/jcs.226852.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
118366Flag-GFP11-pDEST-NBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.
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118367Flag-GFP11-pDEST-CBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.
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118368HA-GFP1-10-pDEST-NBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.
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118369HA-GFP1-10-pDEST-CBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.
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118370HA-GFP1-10-actin-pcDNA4-TOBimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.
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1183712Flag-pDEST-NGateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.
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1183722Flag-pDEST-CGateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.
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1183732HA-pDEST-NGateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.
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1183742HA-pDEST-CGateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.
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118375HA-BirA*-pDEST-N-pcDNA5/FRT/TOExpression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.
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118376pENTR-KAT14-547-782Gateway compatible donor vector with KAT14-547-782 truncation.
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118377pENTR-KAT14-1-546Gateway compatible donor vector with KAT14-1-546 truncation.
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118378pENTR-actinGateway compatible donor vector with WT actin.
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118379pENTR-actin-R62DGateway compatible donor vector with actin-R62D.
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118380pENTR-NLS-actinGateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.
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118381pENTR-NLS-actin-R62DGateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.
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118382KAT14-pDEST10HIS-tagged insect cell expression vector with KAT14.
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118383pENTR-actin-V159NGateway compatible donor vector with actin-V159N.
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118384pENTR-actin-G168D,Y169DGateway compatible donor vector with actin-G168D,Y169D.
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Antibodies from Article