159,481 results

1. SpCas9-NRCH-ABE8e(V106W)

   Plasmid

   #198556

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HDAC7 Flag

   Plasmid

   #13824

   Purpose

   Mammalian expression of human histone deacetylase 7 with flag tag

   Depositor

   Eric Verdin

   Article

   Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

   Insert

   HDAC7 (HDAC7 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hACE2

   Plasmid

   #1786

   Purpose

   Mammalian expression of human ACE2

   Depositor

   Hyeryun Choe

   Article

   Li et al Nature. 2003 Nov 27. 426(6965):450-4.

   Has Service

   Cloning Grade DNA

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pBAC-lacZ

   Plasmid

   #13422

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. luxB:cp157Venus+luxA/pRSETB Duet

   Plasmid

   #197583

   Purpose

   BRET lux luciferase with cp157Venus for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

   Insert

   LuxA LuxBcp157Venus
   

   Use

   E.coli expression

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEIAV-SIN6.1 CGFPW

   Plasmid

   #44171

   Depositor

   John Olsen

   Article

   McKay et al Gene Ther. 2006 Apr;13(8):715-24.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   chromophore mutations

   Promoter

   CMV

   Available since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-4T-1 3xFlag-JNK1a1

   Plasmid

   #47574

   Purpose

   Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   JNK1a1 (MAPK8 Human)
   

   Use

   Tags

   3xFlag and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA4-Chk1-Flag

   Plasmid

   #22894

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Chk1 (CHEK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLL7.0: tgRFPt-SSPB WT

   Plasmid

   #60415

   Purpose

   Mammalian Expression of tgRFPt-Nano

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   tgRFPt-SSPB
   

   Use

   Cre/Lox and Lentiviral

   Tags

   TagRFPt

   Expression

   Mammalian

   Mutation

   Y11K and A15E

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p2CT-His-MBP-Lse_C2c2_WT

    Plasmid

    #83486

    Purpose

    Bacterial expression of WT L. seeligeri C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lse_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3 NT3

    Plasmid

    #39981

    Depositor

    Yves-Alain Barde

    Insert

    Neurotrophin 3 (Ntf3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCS2-Arl13b-mCardinal

    Plasmid

    #194542

    Purpose

    Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST-HisMBP

    Plasmid

    #11085

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac (lactose/IPTG inducible)

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMVTNT-T7-KPNA4

    Plasmid

    #26680

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 . 11():63.

    Insert

    Karyopherin Alpha 4 (KPNA4 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET30a(+)-PE2

    Plasmid

    #180015

    Purpose

    Expressing PE2 protein in E.coli for protein purification

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    PE2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Lac operator-controlled T7 promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Human SERCA2b (pcDNA3.1+)

    Plasmid

    #75188

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    Jonathan Lytton, David MacLennan

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-MET

    Plasmid

    #116762

    Purpose

    Lentiviral expression of MET

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPRyl_A08

    Plasmid

    #84610

    Purpose

    Introduces DSB at A08 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pD649-HAsp-LAIR1-COMP5AP-AviTag-9xHis

    Plasmid

    #157101

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    LAIR1 (LAIR1 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Rasgrf2-2A-dCre targeting vector

    Plasmid

    #61573

    Purpose

    Target a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Rasgrf2-2A-dCre (Rasgrf2 P1 bacteriophage, Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge