pLp_3050sNuc
(Plasmid #122030)

• Purpose: Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei
• Depositing Labs: Vincent Eijsink, Geir Mathiesen
• Publication: Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 10.1186/1471-2164-10-425.

Backbone

• Vector backbone: spp-based expression vector (pSIP401)
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 6188
• Vector type: Bacterial Expression
• Selectable markers: Erythromycin

Growth in Bacteria

• Bacterial Resistance(s): Erythromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: signal peptide Lp_3050
• Species: Signal peptide from Lp_3050 gene in Lactobacillus plantarum
• Insert Size (bp): 124
• Promoter: sppA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: GGCTTTTATAATATGAGATAATGCCGAC

Resource Information

• Supplemental Documents:
  • 3050sNuc.gbk
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid for heterologous secretion of proteins in Lactobacillus plantarum and L. sakei