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MSCV-Cas9-2A-BFP
(Plasmid #164662)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 164662 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV_Cas9_puro
  • Backbone manufacturer
    Addgene 65655
  • Backbone size w/o insert (bp) 10475
  • Total vector size (bp) 10071
  • Modifications to backbone
    PGK promoter and PuroR removed, T2A tagBFP inserted
  • Vector type
    Mammalian Expression, Retroviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Alt name
    S. pyogenes CRISPR-Cas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4101
  • Promoter MSSV_LTR

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTTTATCCAGCCCTCAC
  • 3′ sequencing primer AGCGAGTCAGTGAGCGAG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    BFP
  • Alt name
    blue fluorescent protein
  • Alt name
    TagBFP
  • Species
    Synthetic
  • Insert Size (bp)
    702
  • Promoter MSSV_LTR

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTTTATCCAGCCCTCAC
  • 3′ sequencing primer AGCGAGTCAGTGAGCGAG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene vector 65655

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-Cas9-2A-BFP was a gift from Daniel Hodson (Addgene plasmid # 164662 ; http://n2t.net/addgene:164662 ; RRID:Addgene_164662)
  • For your References section:

    Genetic modification of primary human B cells to model high-grade lymphoma. Caeser R, Di Re M, Krupka JA, Gao J, Lara-Chica M, Dias JML, Cooke SL, Fenner R, Usheva Z, Runge HFP, Beer PA, Eldaly H, Pak HK, Park CS, Vassiliou GS, Huntly BJP, Mupo A, Bashford-Rogers RJM, Hodson DJ. Nat Commun. 2019 Oct 4;10(1):4543. doi: 10.1038/s41467-019-12494-x. 10.1038/s41467-019-12494-x PubMed 31586074