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pET28-his-3C_Tau352
(Plasmid #100092)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100092 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28-his-3C
  • Backbone size w/o insert (bp) 5231
  • Total vector size (bp) 6343
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fetal microtubule-associated protein tau
  • Alt name
    Tau
  • Alt name
    MAP-Tau
  • Species
    H. sapiens (human)
  • Mutation
    wild type sequence
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, tau-40)
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The 3C site is introduced in front of Tau sequence for easy purification by subtractive IMAC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-his-3C_Tau352 was a gift from Nikolai Sluchanko (Addgene plasmid # 100092 ; http://n2t.net/addgene:100092 ; RRID:Addgene_100092)
  • For your References section:

    Bacterial co-expression of human Tau protein with protein kinase A and 14-3-3 for studies of 14-3-3/phospho-Tau interaction. Tugaeva KV, Tsvetkov PO, Sluchanko NN. PLoS One. 2017 Jun 2;12(6):e0178933. doi: 10.1371/journal.pone.0178933. eCollection 2017. PONE-D-16-49989 [pii] PubMed 28575131