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Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion.
Shimatani Z, Kashojiya S, Takayama M, Terada R, Arazoe T, Ishii H, Teramura H, Yamamoto T, Komatsu H, Miura K, Ezura H, Nishida K, Ariizumi T, Kondo A
Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
91692pMonAID_dCas9-PmCDA_Hyg_ALSMonocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls
91693pMonAID_nCas9-PmCDA_Hyg_ALSMonocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls
91694pDicAID_nCas9-PmCDA_NptII_DellaDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella
91695pDicAID_nCas9-PmCDA-2A-NptII_ETRDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Antibodies from Article