Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion.
Shimatani Z, Kashojiya S, Takayama M, Terada R, Arazoe T, Ishii H, Teramura H, Yamamoto T, Komatsu H, Miura K, Ezura H, Nishida K, Ariizumi T, Kondo A
Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. PubMed Article
Shimatani Z, Kashojiya S, Takayama M, Terada R, Arazoe T, Ishii H, Teramura H, Yamamoto T, Komatsu H, Miura K, Ezura H, Nishida K, Ariizumi T, Kondo A
Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 91692 | pMonAID_dCas9-PmCDA_Hyg_ALS | Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls |
| 91693 | pMonAID_nCas9-PmCDA_Hyg_ALS | Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls |
| 91694 | pDicAID_nCas9-PmCDA_NptII_Della | Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella |
| 91695 | pDicAID_nCas9-PmCDA-2A-NptII_ETR | Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr |