166,034 results

1. iRFP670-PIP4K2C

   Plasmid

   #202750

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   iRFP670:PIP4K2C (PIP4K2C Human)
   

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a MDHC_YEAST

   Plasmid

   #204232

   Purpose

   Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHC_YEAST (MDH2 Budding Yeast)
   

   Tags

   6X His

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ1651-sgRNA(F+E)-sgGal4

   Plasmid

   #100549

   Purpose

   sgGal4-expressing vector modified from Addgene plasmid #51024

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Insert

   sgGal4
   

   Use

   CRISPR

   Tags

   sgRNA

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG2-EGFP-C-IFT144

   Plasmid

   #218740

   Purpose

   Expresses C-terminally EGFP-tagged IFT144 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Insert

   IFT144 (WDR19 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2854

   Plasmid

   #143384

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FEV (FEV Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-VEGFR2-V5/HIS

   Plasmid

   #205617

   Purpose

   Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR2 receptor tyrosine kinase (KDR Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

   Plasmid

   #220610

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-HA-USP33

   Plasmid

   #22601

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP33 (USP33 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-mE1

   Plasmid

   #32534

   Depositor

   Jorge Eduardo Azevedo

   Article

   Carvalho et al Mol Biotechnol. 2011 Oct 20.

   Insert

   E1, ubiquitin-activating enzyme (Uba1 Mouse)
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pThermoCas9_ctrl

    Plasmid

    #100981

    Purpose

    Expresses ThermoCas9 and its sgRNA module

    Depositor

    John van der Oost

    Article

    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

    Inserts

    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    

    Tags

    None

    Expression

    Bacterial

    Promoter

    B. coagulans DSM 1 pta promoter and B. smithii xy…

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSNAPf-N1

    Plasmid

    #58187

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    SNAP tag

    Expression

    Mammalian

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pENTR223-BcLF1

    Plasmid

    #198581

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BcLF1
    

    Use

    Gateway entry vector

    Mutation

    None (wildtype)

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-CjCas9

    Plasmid

    #89754

    Purpose

    Expression of CjCas9 with His tag in E.coli

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    6XHis, HA, and SV40 NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR223-BXLF2

    Plasmid

    #195830

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BXLF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BXLF2 (BXLF2 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHIV-Zsgreen

    Plasmid

    #18121

    Depositor

    Bryan Welm, Zena Werb

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Insert

    Zsgreen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSG5-BBLF4

    Plasmid

    #72634

    Purpose

    Mammalian expression vector for Epstein-Barr virus DNA helicase

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BBLF4
    

    Expression

    Mammalian

    Mutation

    P5L

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pAW63.YY1.FKBP.knock-in.BFP

    Plasmid

    #104371

    Purpose

    For knocking in FKBP F36V P2A BFP

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    FKBP12(F36V) -P2A-BFP
    

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE-60 roGFP2-Orp1-His

    Plasmid

    #64976

    Purpose

    Bacterial expression of His-tagged roGFP2-Orp1

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    His and roGFP2

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGEN-dPspCas13b-NES-mCherry

    Plasmid

    #233609

    Purpose

    Expression of catalytically-inactive PspCas13b in mammalian cells

    Depositor

    Shintaro Iwasaki

    Article

    Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

    Insert

    dPspCas13b-NES-mCherry
    

    Use

    CRISPR

    Tags

    3xFlag and mCherry

    Mutation

    H133A/H1058A

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-GRAM1b

    Plasmid

    #211494

    Purpose

    Expression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Naito et al Elife. 2019 Nov 14;8. pii: 51401. doi: 1

    Insert

    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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