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pSEZ-1
(Plasmid #51868)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51868 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSDH2(P-2)GFP
  • Backbone manufacturer
    B. D. Lemire
  • Backbone size w/o insert (bp) 3470
  • Modifications to backbone
    pSDH2(P-2)GFP contains the promoter and the mitochondrial localization sequence of SDHB-1 inserted into the vector pPD95.79. pSEZ-1 replaces the GFP with the cpYFP coding sequence amplified by PCR from pShuttle–CMV-mito-cpYFP.
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    psdhb-1::mtLS-cpYFP
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    178
  • Promoter sdhb-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13-Rev
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEZ-1 was a gift from Meng-Qiu Dong (Addgene plasmid # 51868 ; http://n2t.net/addgene:51868 ; RRID:Addgene_51868)
  • For your References section:

    Mitoflash frequency in early adulthood predicts lifespan in Caenorhabditis elegans. Shen EZ, Song CQ, Lin Y, Zhang WH, Su PF, Liu WY, Zhang P, Xu J, Lin N, Zhan C, Wang X, Shyr Y, Cheng H, Dong MQ. Nature. 2014 Feb 12. doi: 10.1038/nature13012. 10.1038/nature13012 PubMed 24522532