166,034 results

1. ptf-Galectin3

   Plasmid

   #64149

   Purpose

   Expression in mammalian cells

   Depositor

   Tamotsu Yoshimori

   Article

   Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

   Insert

   Galectin3 (LGALS3 Human)
   

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-fDIO EYFP

   Plasmid

   #55641

   Purpose

   Flp-Dependent EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTU-DO-LEU-C4

   Plasmid

   #160341

   Purpose

   High-copy expression vector for K. marxianus, leucine selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Expression

   Yeast

   Promoter

   n/a

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSG5-BALF2

   Plasmid

   #72629

   Purpose

   Mammalian expression vector for Epstein-Barr virus single-strand DNA binding protein

   Depositor

   S. Diane Hayward

   Article

   Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

   Insert

   BALF2
   

   Expression

   Mammalian

   Mutation

   S1028P and V1088A

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-dCas13-inactive M3nls

   Plasmid

   #157854

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

   Plasmid

   #65429

   Purpose

   Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Mutation

   H134R variant

   Promoter

   hSyn1

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMX229

   Plasmid

   #23002

   Depositor

   Linda Wordeman

   Article

   Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

   Insert

   Kif18A (KIF18A Human)
   

   Tags

   6His and EGFP

   Expression

   Mammalian

   Available Since

   Feb. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. gag-MCP-pol

   Plasmid

   #211370

   Purpose

   Packaging plasmid for v3 PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-MCP-pol
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3

   Plasmid

   #200630

   Purpose

   Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   iCasp9
   

   Use

   Lentiviral

   Promoter

   pTRE3G

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNF02-mLemon

    Plasmid

    #219399

    Purpose

    Constitutive expression of mLemon in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mLemon
    

    Expression

    Bacterial

    Promoter

    proDp

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-mROCK2

    Plasmid

    #101296

    Purpose

    Mammalian expression of Rock2-GFP

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock2 (Rock2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221_SLC38A10

    Plasmid

    #132083

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A10 (SLC38A10 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid

    #36047

    Purpose

    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA(R118G)-HA
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Available Since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b RNase A

    Plasmid

    #58903

    Purpose

    Bacterial expression of bovine pancreatic ribonuclease A

    Depositor

    Ronald Raines

    Article

    delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

    Insert

    RNase A (RNASE1 Bovine)
    

    Tags

    pelB sequence

    Expression

    Bacterial

    Mutation

    See Depositor Comments below

    Promoter

    T7

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. TTK

    Plasmid

    #38907

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TTK (TTK Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pNIC28-DENV3-NS5FL

    Plasmid

    #86013

    Purpose

    Expression of dengue virus 3 NS5 protein

    Depositor

    Dahai Luo

    Article

    Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

    Insert

    DENV3-NS5FL
    

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    amino acid residues 6 to 895 of DENV3 NS5

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti PGK FKBP12(F36V)-OGT (Neo)

    Plasmid

    #154294

    Purpose

    Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC-9-OsAct2-11

    Plasmid

    #197765

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    OsAct2 terminator
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMC-9-ZmUbi-11

    Plasmid

    #197777

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmUbi terminator
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-HA-JMJD2B

    Plasmid

    #24181

    Purpose

    Mammalian expression of human JMJD2B with HA tag

    Depositor

    Kristian Helin

    Article

    Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

    Insert

    JMJD2B (KDM4B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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